GA/T 148-2019 in English
VALIDForensic medicine—Specifications for specimen collection,fixation,sampling and preservation
- Issued on:2019-10-14
- Implemented on:2019-12-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$156.00
本标准规定了法医学中病理检材的提取、固定、包装、送检、取材及保存的原则、内容和方法。
本标准适用于各级公安、检察机关、医学院校及面向社会服务的鉴定机构所进行的法医病理学检验。
Introduction
Core changes in the standard revision
| Technical elements | 1996 edition | XXXX edition | Main points of change |
|---|---|---|---|
| Standard structure | Chapter 7 | Chapter 9 | New chapter on specimen preservation (Chapter 9) |
| Fixative solution specifications | Only formalin is specified | Neutral formalin configuration is added | pH value control requirements (7.0-7.4) |
| Specimen preservation period | Undefined | 12-month benchmark | Strengthening legal risk prevention and control |
Analysis of key technical points
1. Three-dimensional specification for specimen extraction
Chapter 4 of the standard clearly requires: Injury specimens must carry adjacent normal tissues, including:
- Current spot: When the diameter is greater than 4cm, strips of tissue must be taken across the maximum diameter
- Neck cord groove: Longitudinal incision perpendicular to the cord groove
- Bone marrow extraction: For hematopoietic system diseases, more than 3 sites must be extracted (ilium/sternum/femur)
2. Scientific ratio of fixative
Chapter 5 of the standard specifies two core fixatives:
- Conventional 10% formalin: formaldehyde: water = 1:9 (prepared and used immediately)
- Neutral buffered formalin: add phosphate buffer (pH7.0-7.4)
Typical case: Acidic formalin fixation of bleeding foci is prone to produce brown-black precipitates, and the neutral formula can effectively avoid this phenomenon.
Special requirements for organ processing
Cardiac conduction system sampling
Standard 8.1.3 stipulates:
- Sinoatrial node: 3-4cm tissue longitudinally cut at the junction of the superior vena cava and right atrium
- Atrioventricular bundle: The vertical section on the left side of the coronary sinus includes the atrial septum and ventricular septum
Standardized brain cutting method
The 12-cut 13-piece coronal cutting method is adopted:
- 4th cut: sampling from the middle frontal gyrus/cingulate gyrus
- 7th cut: sampling from the mammillary body
- 8th cut: double sampling of the hippocampus/thalamus
Implementation suggestions
- Sample container management: Special containers with a diameter of ≥30cm and a height of ≥32cm. Mixing of samples from multiple cases is prohibited
- Infectious disease prevention and control: HIV and other positive samples must be marked with a red label and stored separately
- Sample packaging: Long-distance transportation requires 3-4 layers of formalin gauze + sealed plastic bag + hard outer box

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