GB/T 15670.10-2017 in English

VALID

Toxicological test methods for pesticides registration- Part 10:Short-term repeated dose 28-day oral toxicity study

Issued on:2017-07-12
Implemented on:2018-02-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $120.00
$117.00

Standard No:	GB/T 15670.10-2017
Document status:	VALID
Title in English:	Toxicological test methods for pesticides registration- Part 10:Short-term repeated dose 28-day oral toxicity study
Title in Chinese:	农药登记毒理学试验方法第10部分：短期重复经口染毒(28天)毒性试验
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-07-12
Implemented on:	2018-02-01
ICS Classification:	65.100-Pesticides and other agrochemicals
Chinese Classification:	B17-Pesticide management and application method
Professional Classification:	GB-National Standard
Related Keywords:	toxicological test methods toxicity test analysis toxicity test dose 28-day oral toxicity study introduction gb/t15670.10-2017 short-term repeated oral exposure
Related Topics:	toxicology toxicity Pesticide Registration toxic reaction Toxicology+ Toxicity test Pesticide Toxicity Test Toxicity verification reaction toxicity Pesticide Registration Toxicology Test Unit repeated toxicity test t Toxicity Toxicity marker 14 Toxicology test Toxicology test Pesticide Toxicology Test Toxicity standard Pesticide Registration Pesticide Registration Toxicology Test Pesticide Toxicology Test Methods Pesticide Registration Toxicology Test Pesticide Registration Toxicology Test Unit Toxicology test methods for pesticide registration Phase IV toxicity, long-term toxicity Carbon 10 toxicity poisoned Toxicity test repeat toxicity test Toxicity Test Drugs Pesticide Registration Toxicology repeat exposure toxicity GB/T 15670.10 GB/T 15670.10-2017 local toxicity test eb dye toxicity Pesticide 1065 toxicity Toxicology Experimental Methods and Techniques Violent poisoning method Method verification repeatability Retest requirements for chemical retest period Electronic balance repeatability test method New techniques and methods in toxicology Moisture method repeatability Common methods for long-term toxicity test dose design Repeatable method validation Dissolution Methodology Validation Repeatability

《GB/T 15670.10-2017农药登记毒理学试验方法第10部分：短期重复经口染毒（28天）毒性试验》由326（农业农村部）归口，主管部门为农业农村部。

Introduction

GB/T15670.10-2017: Interpretation of short-term repeated oral exposure (28 days) toxicity test

Analysis of standard formulation background and technological evolution

GB/T15670 "Toxicological Test Methods for Pesticide Registration" series of standards is an important basis for pesticide registration evaluation in my country. Part 10 of this revision is mainly for short-term repeated oral exposure (28 days) toxicity test.

Old version of the standard	New version of the standard	Main changes
GB/T15670-1995	GB/T15670.10-2017	Structural optimization, new content (Chapter 1, Chapter 2, Chapter 3, Chapter 5, Chapter 6.1, Chapter 7 and Chapter 8)
Requirements for experimental animals are not specified in detail	It is clearly stated that healthy young adult rats should be used, half male and half female (at least 10 in each group)	Specific requirements for experimental animals are added
Dosage design principles are not specified	Set up three dose groups and one control group. The high dose should show obvious toxicity but not lethal.	Optimize dose grouping rules

Comparison of standard framework and analysis of key technical points

The main purpose of this standard is to evaluate the toxic effects of pesticides on animals through repeated oral exposure in the short term, determine the no observed adverse effect level (NOAEL) and the lowest observed adverse effect level (LOAEL), and provide a basis for subsequent subchronic and chronic toxicity tests.

Key technologies	Specific requirements	Actual application cases
Selection of experimental animals	Healthy young adult rats, half male and half female, at least 10 in each group	For example, when evaluating a certain herbicide, 6-week-old C57BL/6 mice are used for the test.
Route of administration	Mixed into feed or drinking water, or by gavage	For pesticides that are poorly soluble in water, edible oil (such as olive oil) is usually used to prepare the solution.
Observation indicators	Include body weight, food intake, hematological indicators (hemoglobin, white blood cell count, etc.), blood biochemical indicators (ALT, AST, BUN, etc.) and histopathological examination.	For example, a pesticide showed abnormal liver function in the high-dose group, and its relationship with poisoning needs to be further analyzed.

Implementation suggestions

Experimental design: Ensure that the experimental animals meet the standard requirements. It is recommended to use standardized mouse strains such as C57BL/6.
Dose preparation: Pay attention to the uniformity and stability of the test substance to avoid affecting the test results due to palatability issues.
Data statistics: It is recommended to use professional software such as SPSS or GraphPad Prism for data analysis, and focus on the analysis of NOAEL and LOAEL values.
Follow-up research: According to the results of this test, when designing subchronic toxicity tests, it is necessary to focus on the pathological changes of target organs such as the liver and kidneys.

More samples: QC/T 942-2021 JC/T 2453-2018 JJG 21-2008

Sample only — not a preview of GB/T 15670.10-2017

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