GB/T 15670.17-2017 in English

VALID

Toxicological test methods for pesticides registration. Part 17: Mammalian spermatogonial/spermatocyte chromosome aberration test

Issued on:2017-07-12
Implemented on:2018-02-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 15670.17-2017
Document status:	VALID
Title in English:	Toxicological test methods for pesticides registration. Part 17: Mammalian spermatogonial/spermatocyte chromosome aberration test
Title in Chinese:	农药登记毒理学试验方法第17部分：哺乳动物精原细胞/精母细胞染色体畸变试验
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-07-12
Implemented on:	2018-02-01
ICS Classification:	65.100-Pesticides and other agrochemicals
Chinese Classification:	B17-Pesticide management and application method
Professional Classification:	GB-National Standard
Related Topics:	cell alpha cells multicellular animal sporogenous cells cell chromosomal aberration collagen and cells Mycoplasma cells cell staining cell staining biological cell water mycoplasma dna cell method cell principle cellular contaminants cell english Chromosomal aberration assay in mouse spermatogonia or spermatocytes cell contamination cell night Cytoplasma Labeled cell analysis stained cells cell pretest Cellfood Cellfood Accurate Cell Counting mammalian sperm cells stained cells primary cell staining Cellular Collagen Analysis cell dose primary cell staining cell contamination if cell Calcium cells cells that can be stained Double Antibody in Cell Assay Cell Stainer Accurate Cell Counting Method cell transient principle cell block cell physics mammalian cell collagenase cells Principle of cell line cell test data cells are stained Cell Variation cell damage cells below zero cell standard Bladder cancer cell chromosomes cell test tube cells in situ mycoplasma cell method electrocytometry cells in situ Cell experiment generations of cells accurate cell count Mycoplasma deulens Principle of c6 cytometer cell version test tube cells food cell food cell Cell + Bacteria cell in situ method oriental cells Mycoplasma target cells Cytometer principle cell version mtt cell extracellular cells mtt+ cells cell staining Raw Cell Organic Food Dye-based cell detection Cell Staining Before Flow Cytometry testicular cells Flow Cytometry Intra-Staining parietal cell Organelle approach cell deformation Cell experiment method chromosomal cells long-term cellular approach how to label cells protopic cells Cell count test method Toxicology test pesticide cell time animal cells transient cell Mycoplasma cytoplasma cell determination Principles of Cell Assays cell count test sperm cells sperm cells Cell Compound animal cell experiments Toxicology test animal cell method primary cells Automatic fine Cytometer principle Cell Stainer Cell Stainer Cytometer principle sperm cells Cell Collagen Experiment cell nudity How many generations of cells are used in general experiments "cell" cellular machinery animal cell tank Cytology Abroad Pesticide Registration Toxicology Test Pesticide Toxicology Test Methods Cell double staining Pesticide Registration Toxicology Test cell count test tuft cells cell english cell detection test The principle of instant cell lysis Type II Cytotoxicity Test parietal cell method Intracellular flow staining double stained cells double stained cells Toxicology test methods for pesticide registration Principle of flow cytometry Principle of automatic cytometer cell staining Indirect staining for flow cytometry acellular animal Principle of High Frequency Cytometer Automated Cell Processing precision cells Mycoplasma cells cellular means cell membrane method How L6 cells differentiate leukocyte 17 cellular transient principle testicular cells How to Double Stain Cells cell mycoplasma removal multicellular animals Cellular Level and Animals cell if Sporocyte animal cell coat Three staining of cells Cytotoxic Drugs Laboratory intracellular drug Principle of cell nucleus double staining of cells primary cell animal mouse spermatocytes cell animal experiments cell + version unstained cells androgynocyte spermatogonia How to measure animal cells cell deformation sperm cell chain Bromoblasts stained cells sex blast cell experiments flow cytometry staining primary sperm cells Transient labeling of cells cell staining GBT15670.17 GB/T 15670.17-2017 Principle of series excitation test live cell labeling plasmablast b cells Principle of TRAP staining of osteoclasts cell products Experimental principles of cell passaging Principles of cell passaging experiments Cell experiment methods Pharmacological cell experiments Manifestations of cells contaminated by mycoplasma Mycoplasma contaminated cells tetracycline Twins xy chromosomes plasma cell surface markers Principles for dosing drug-resistant cells Principles of cytotoxic drugs Rules for dosing drugs in cell experiments Principles of neutrophil isolation trap cell staining Phase separation experiments in living cells Single cell memory t cell marker lipo2000 transfected cell lines adherent cells Principle of detecting cell apoptosis by caspase3 Cell recovery principle caco-2 cell contamination Methods and principles of cell lysis Cytotoxic T cell differentiation process transfection of jurkat cells Principle of spleen cell proliferation experiment Cell experiment drugs are not soluble in DMSO Materials live and dead cell staining methods Cytotoxicity test to determine cytotoxicity Methods to remove mycoplasma cells Methods for breaking animal cell membranes Cell starvation treatment method Cell biology detection methods Which method is used to stain cultured muscle cells? Cell fixation method Cell crystal violet staining method Cell membrane disruption method Fixed cell method Cell methodology validation Physical adsorption method of immobilized cells Overview of synthesis methods for cytotoxic drugs Commonly used cell differentiation methods Methods for isolating cytoplasm and nuclei Evaluating Drug Cytotoxicity Principles of gentle method to lyse cell nuclei Mycoplasma cell treatment methods Animal cell fusion methods Principle of cell membrane extraction method Flow cytometry methodology validation Methods for detecting mycoplasma contamination in cells Methods for isolating animal cells Neutrophil staining method Animal cell membrane disruption method Mechanical methods for dispersing animal cells Intracellular drug concentration verification method Cell organelle fluorescent labeling method Cell biochemical staining methods Cell smear method Intracellular ferritin staining method Cell fixation method Cell cycle statistical analysis methods Methods to promote animal cell fusion Methods for extracellular electrophysiological recording Mammalian cell disruption methods Mammalian cell culture media industry Cytological assays Leukocyte differential staining method Chlamydia cell discoloration culture method ultrasonic cell method How to stain cells with trypan blue Methods for introducing plasmids into animal cells Experimental Method for Cell Thermal Displacement Determination Methods for mechanically obtaining single animal cells Animal cell fusion methods chemical methods of cell fusion Cell immunostaining method Methods for cell transfection with lentivirus Bioinformatics analysis methods for single-cell sequencing Cell staining industry Cell Thermal Displacement Experimental Method Cell membrane fusion method Cell tumorigenicity test methods Methods and principles of cell nucleus extraction Flow cytometry methodology validation How to observe cell protoplasts Advanced methodology of transfection cell method Cell viability detection methods and principles Sperm cell isolation method Principles and methods of Wright's staining Cell trypan blue staining method Cellular iron staining methods Methods for dispersing animal cells cell lysis methods chemical methods Mycoplasma cell detection test method Cell transfection procedures Trypan blue cell staining and counting method Cell extract preparation method Staining method cells Common cell staining methods Nucleus staining method How to split cells Specific methods of cell thermal displacement Animal cell fixation methods Cell thermal shift assay experimental method Cell invasion assay staining method Specific configuration of cytotoxic drugs Common animal cell fixation methods Principles and methods of cell fusion Cell engineering principles and methods Method for regenerating plant protoplast cell wall Cell chromosome separation method Cell chromosome extraction method Red blood cell staining method The most common cytochemical staining methods Liquid-based cell processing method Large-scale culture method of mammalian cells Cell transfection method There are several cell staining methods. Animal cell protoplast method Cell synchronization treatment method Chloroplast and cell isolation methods Mammalian cell transfection methods

《GB/T 15670.17-2017农药登记毒理学试验方法第17部分：哺乳动物精原细胞/精母细胞染色体畸变试验》由326（农业农村部）归口，主管部门为农业农村部。

Introduction

GB/T15670.17-2017 Standard Overview

This standard specifies the basic principles, methods and requirements for mammalian spermatogonia/spermatocyte chromosome aberration tests, and is applicable to the evaluation of the possibility of test substances causing genetic mutations in germ cells during the pesticide registration process.

Comparison of standard frameworks

Old version GB/T15670-1995	New revised content	Update highlights
Test name unclear	Changed to “Mammalian spermatogonia/spermatocyte chromosome aberration test”	Test name standardized
Structure and layout format are not unified	The overall structure of the standard has been adjusted, and chapter content has been added (Chapter 1 to Chapter 5, 7.1, Chapter 9)	Readability and standardization have been improved
Animal requirements and dose groupings are not clarified	The animal requirements (see 7.2), dosage and grouping requirements (see 7.3) have been modified.	The test conditions are more stringent.

Explanation of professional terms and practical application cases

Chromosome structural aberration:refers to the structural changes in the metaphase of cell division observed under a microscope, such as chromosome deletion, exchange and internal exchange.

Gap:refers to the damage length of chromosomes and chromatids that is less than the width of a chromatid, which is the smallest form of incorrect arrangement.

For example, if a significant increase in chromosome aberrations or gaps is observed in the mitotic cells in the high-dose group in the experiment, it can be judged that the test substance has a potential mutagenic effect.

Implementation recommendations

Experimental design:A preliminary test should be conducted before the formal test to determine the appropriate dosage range, especially for test substances that are insoluble in water or oil, which need to be prepared with a suspension.
Control group setting:In addition to the negative control group, it is recommended to set up a positive control group (such as cyclophosphamide and mitomycin C) to verify the effectiveness of the test system.
Data analysis:In statistical analysis, in addition to paying attention to the rate of aberrant cells, the dose-response relationship must also be examined. If there is a significant difference and there is a clear dose-dependence, it can be determined as a positive result.
Record preservation:It is recommended to preserve all test data for a long time and ensure that the original records are traceable.

More samples: JGJ 94-2008 DL/T 368-2010 NB/T 47013.2-2015

Sample only — not a preview of GB/T 15670.17-2017

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