GB/T 15805.2-2017 in English
VALIDDiagnosis of infectious haematopoietic necrosis
- Issued on:2017-11-01
- Implemented on:2018-05-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 15805.2-2017传染性造血器官坏死病诊断规程》由TC156(全国水产标准化技术委员会)归口,TC156SC11(全国水产标准化技术委员会水产养殖病害防治分会)执行,主管部门为农业农村部。
Introduction
Revision Background and Significance of the National Standard of the People's Republic of China GB/T 15805.2—2017
GB/T 15805.2—2017 "Diagnostic Procedure for Infectious Hematopoietic Necrosis" is an important standard in the aquatic field, replacing the previous GB/T 15805.2—2008. The new standard mainly optimizes the name modification, update of detection methods and new immunological detection technology (such as enzyme-linked immunosorbent assay and indirect immunofluorescence antibody test) to make the diagnosis more accurate.
Comparison of diagnostic technologies for infectious hematopoietic necrosis
| Name | Old method | New method | Scope of application |
|---|---|---|---|
| Virus isolation and culture | PCR amplification | Cell isolation and culture combined with RT-PCR, ELISA and IFAT | Applicable to the detection of clinical samples |
| Molecular detection technology | Single PCR method | Combination of multiple detection technologies | Precise laboratory diagnosis |
| Immunological detection | - | New ELISA and IFAT methods | Rapid screening and confirmation |
Implementation suggestions and precautions
Laboratory conditions requirements:A biosafety level 2 laboratory is required to ensure adequate protection for testing personnel. Instruments and equipment include fluorescence microscopes, PCR instruments, and microplate readers.
Key points for sample collection:Sampling should be carried out in a low temperature environment, fish with clinical symptoms should be given priority, and samples should be processed according to standard specifications to ensure detection accuracy.
Method selection:Depending on laboratory conditions and testing requirements, RT-PCR can be used as a preliminary screening method, and ELISA or IFAT can be combined for confirmation when necessary.

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