GB/T 31810-2015 in English

VALID

Detection and identification of Maize chlorotic mottle virus

Issued on:2015-07-03
Implemented on:2015-11-27
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $250.00
$243.00

Standard No:	GB/T 31810-2015
Document status:	VALID
Title in English:	Detection and identification of Maize chlorotic mottle virus
Title in Chinese:	玉米褪绿斑驳病毒检疫鉴定方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2015-07-03
Implemented on:	2015-11-27
ICS Classification:	65.020.01-Farming and forestry in general
Chinese Classification:	B16-Plant quarantine, pest control
Professional Classification:	GB-National Standard
Related Keywords:	maize chlorotic mottle virus chlorotic mottle virus introduction detection methods detection method sensitivity scope real-time fluorescence rt-pcr quantitative detection suitable main detection methods
Related Topics:	virus+ hosta virus hosta x virus Quarantine and identification method of canna yellow mottle virus Quarantine and identification method of maize chlorotic mottle virus Quarantine and identification method of corn chlorotic dwarf virus virus standard Canna yellow mottle virus Quarantine identification of canna yellow mottle virus Absolute quantification of virus detection full wave mottled corn chlorotic mottle virus poison test virus way Plant virus identification method Mottled world Prion method Prion Quarantine GBT31810 GB/T 31810-2015 Virus single spot screening R virus Calibration method of corn weigher Pseudovirus purification method The old-fashioned way to test corn moisture National standard testing method for corn test weight Virus checking methods Quarantine and identification method of iris yellow spot virus Virus sequencing identification and judgment Corn steep liquor dissolved phosphorus detection method Specific identification methods of virus species

《GB/T 31810-2015玉米褪绿斑驳病毒检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
本标准规定了玉米褪绿斑驳病毒检疫鉴定的方法。
本标准适用于可能携带玉米褪绿斑驳病毒的种子、种苗等植物繁殖材料中玉米褪绿斑驳病毒的检疫鉴定。

Introduction

1. Standard Overview

This standard specifies the quarantine identification method for Maize Chlorotic Mottle Virus (MCMV), and is applicable to plant propagation materials such as seeds and seedlings that may carry the virus. The main detection methods include double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), reverse transcription polymerase chain reaction (RT-PCR) and real-time fluorescence RT-PCR.

2. Detection Principle

The molecular biological characteristics, serological characteristics and biological characteristics of Maize Chlorotic Mottle Virus are the main basis for the formulation of this standard. By detecting the nucleic acid or antigen of the virus, it can be effectively determined whether the sample carries the virus.

3. Comparison of detection methods

Detection method	Sensitivity	Scope of application	Operation steps
DAS-ELISA	High sensitivity	Suitable for preliminary screening	1. Coating antibody2. Sample addition detection3. Enzyme-labeled antibody incubation4. Color reaction
RT-PCR	Highly specific	Suitable for amplification detection of viral nucleic acid	1. RNA extraction2. cDNA synthesis3. PCR amplification4. Electrophoresis analysis
Real-time fluorescence RT-PCR	Quantitative detection	Suitable for accurate diagnosis	1. RNA extraction2. cDNA synthesis3. Real-time amplification monitoring

4. Implementation suggestions

Case study:In actual applications, DAS-ELISA is recommended as a preliminary screening method, and positive samples are further confirmed by RT-PCR or real-time fluorescence RT-PCR. It is recommended to establish standardized operating procedures and regularly train testing personnel.

More samples: JGJ 94-2008 JJG 21-2008 GB 50021-2001(2009)

Sample only — not a preview of GB/T 31810-2015

Loading PDF document...

Error loading PDF. Please make sure the file is valid and try again.

We also recommend

GB/T 44619-2024 in English

Detection and identification of Pomacea Perry
2024-09-29
GB/T 23416.1-2009 in English

Safety technical specification of pest control for vegetables—Part 1:General principles
2009-03-28
GB/T 23617-2009 in English

General principles of survey on forestry quarantine pest
2009-04-27
GB/T 32717-2016 in English

Detection and identification of Toxotrypana curvicauda Gerstaecker
2016-06-14
GB/T 39916-2021 in English

Guidelines for the establishment of plant quarantine system in entry and exit container depot
2021-04-30
GB/T 15791-2011 in English

Rules of monitoring and forecasting for the rice sheath blight (Rhizoctonia solani Kukn)
2011-09-29
GB/T 24829-2009 in English

Identification of Trichodorus species as virus vectors for import and export quarantine
2009-12-15
GB 7411-2009 in English

Quarantine protocol for cotton seeds in producing areas
2009-04-27