GB/T 32226-2015 in English

VALID

Specific solid enzyme(ALD,TIM,GDH)reagent for determination of fructose diphosphate(FDP)

Issued on:2015-12-10
Implemented on:2016-07-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 32226-2015
Document status:	VALID
Title in English:	Specific solid enzyme(ALD,TIM,GDH)reagent for determination of fructose diphosphate(FDP)
Title in Chinese:	果糖二磷酸钠（FDP）含量测定用固体复合酶（ALD、TIM、GDH）试剂
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2015-12-10
Implemented on:	2016-07-01
ICS Classification:	71.040.30-Chemical reagents
Chinese Classification:	G66-Biochemical reagent and clinical analysis reagent
Professional Classification:	GB-National Standard
Related Topics:	GB/T 32226-2015 ald tim GB/T32226-2015 gb32226 gb/t32226 ald+ fdp Enzyme reagent Compound content sodium fructose diphosphate reoxygenated sugar Phosphatase test Phosphorus content of the second class water quality GBT32226 GB/T 32226-2015 multienzyme complex Fructose 6 Phosphate N-glycan complex type What is the solid content test of water reducing agent? gbt297 32226

《GB/T 32226-2015果糖二磷酸钠（FDP）含量测定用固体复合酶（ALD、TIM、GDH）试剂》由TC387（全国生化检测标准化技术委员会）归口，主管部门为国家标准化管理委员会。
本标准规定了果糖二磷酸钠(FDP)含量测定用固体复合酶(ALD、TIM、GDH)试剂的技术要求、试验方法、检验规则、标志、包装和贮存。
本标准适用于果糖二磷酸钠(FDP)含量测定用固体复合酶(ALD、TIM、GDH)试剂。

Introduction

Interpretation of the National Standard of the People's Republic of China GB/T32226-2015

Standard Dimensions	Technical Requirements	Test Methods	Inspection Rules
Composition	Ald (aldolase), Tim (triosephosphate isomerase), Gd h (glycerolphosphate dehydrogenase), the weight ratio of each component is 20%-25%, 30%-40%, 30%-35%	Use UV-visible spectrophotometry to determine the absorbance change of reduced coenzyme I at 340nm	Factory inspection items include appearance and enzyme activity; type inspection is a full inspection
Physical and chemical requirements	The activity of solid composite enzyme is not less than 40μg/(min·mg)	Use triethanolamine hydrochloride buffer (pH7.6) to prepare the solution and measure the absorbance change	The sampling rules shall comply with HG/T3921 regulations
Sensory requirements	Light red or off-white lyophilized powder, dissolved in tris(hydroxymethyl)aminomethane solution (pH7.6)	-	-

Standard formulation background and technology evolution analysis

Background: Fructose diphosphate sodium (FDP) is an important biochemical reagent widely used in the medical field. Its content determination requires accurate enzyme activity analysis.

Technological evolution: From traditional spectrophotometry to modern UV-visible spectrophotometry, the detection accuracy and efficiency have been significantly improved. The complex enzyme system in the standard optimizes the reaction conditions and improves the detection sensitivity.

Implementation suggestions

Laboratory conditions: Equipped with UV-visible spectrophotometer, temperature control equipment, etc.
Staff training: Operators must undergo professional training and be familiar with the standard content and experimental methods
Quality control: Sampling and inspection must be carried out strictly in accordance with the standard requirements to ensure product quality

More samples: QC/T 942-2021 JJG 21-2008 DL/T 368-2010

Sample only — not a preview of GB/T 32226-2015