GB/T 33127-2016 in English
VALIDDetection and identification of Sugarcane streak virus
- Issued on:2016-10-13
- Implemented on:2017-05-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 33127-2016甘蔗线条病毒检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
1. Background and significance of the standard
GB/T33127-2016 "Quarantine and Identification Method for Sugarcane streak virus" is the first standardized detection method for sugarcane streak virus (SSV) in my country. The standard was proposed and coordinated by the National Technical Committee for Plant Quarantine and Standardization, and the main drafting units were the Guangdong Entry-Exit Inspection and Quarantine Bureau and the Hainan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
2. Detection principle and method
| Detection method | Principle description | Applicable scenarios |
|---|---|---|
| PCR detection | Identification is carried out by amplifying SSV-specific gene fragments. | Applicable to precise detection in the laboratory stage. |
| DAS-ELISA method | Uses double antibody sandwich enzyme-linked immunosorbent assay technology to detect viral antigens. | Suitable for rapid initial screening and quantitative analysis. |
| Real-time fluorescence PCR | Based on real-time fluorescence signal monitoring of the amplification process, high-sensitivity detection is achieved. | Suitable for accurate detection of trace samples. |
| Immunoelectron microscopy | Observes the morphological characteristics of virus particles through an electron microscope. | Used for virus morphology research and auxiliary diagnosis. |
3. Laboratory equipment and reagent requirements
Laboratory testing requires a variety of precision instruments, including PCR instrument, real-time fluorescence quantitative PCR instrument, transmission electron microscope, etc. All reagents must meet analytical purity standards, and experimental water must meet the requirements of GB/T6682.
4. Testing process and result judgment
The testing process includes symptom observation, sampling, laboratory testing (PCR, DAS-ELISA, etc.) and comprehensive result judgment. The sample can be judged as positive if it meets one of the following conditions: PCR test is positive and the sequence is consistent, or at least one of the three test methods is positive.
5. Comparison and optimization suggestions of standard frameworks
| Standard dimensions | Existing framework | Improvement suggestions |
|---|---|---|
| Detection sensitivity | The PCR method has high sensitivity, but takes a long time. | It is recommended to introduce rapid detection technology to improve efficiency. |
| Reagent standardization | The source of reagents must be indicated, and some reagents rely on imports. | Promote the research and development and application of domestic reagents. |
| Training system | Detection personnel must undergo professional training. | Establish a standardized training system and conduct technical exchanges on a regular basis. |
6. Implementation Suggestions
To ensure the effective implementation of the standard: strengthen the construction of the testing team and improve the laboratory hardware conditions; optimize the testing process and improve the testing efficiency; promote international cooperation and establish a regional virus information sharing mechanism.
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