GB/T 35331-2017 in English
VALIDDetectian and identification of Didymella lycopersici Klebahn
- Issued on:2017-12-29
- Implemented on:2018-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$243.00
《GB/T 35331-2017番茄亚隔孢壳茎腐病菌检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
Overview of the Standard for Quarantine Identification Methods for Tomato Didymella Klebahn
Tomato Didymella Klebahn (Didymella lycopersici Klebahn) is a quarantine disease that seriously affects tomato cultivation. This standard specifies the quarantine identification methods for the pathogen in detail, providing an important technical basis for agricultural production.
Background of Standard Formulation
With the acceleration of globalization, the risk of agricultural disease transmission has increased. GB/T 35331-2017 was formulated based on domestic and foreign research results, aiming to unify the identification process of tomato Didymella Klebahn to prevent the disease from spreading through seeds and plants.
Detailed explanation of identification method
| Steps | Specific requirements | Precautions |
|---|---|---|
| Selection of suspected materials | Select shriveled, deformed seeds and diseased residues as test samples | Ensure the operating environment is sterile to avoid cross contamination |
| Isolation and culture | Use PDA, MEA and OA culture media for separation | Control the culture temperature at 18-20℃ and maintain alternating light |
| Analysis of morphology and metabolic characteristics | Observe the characteristics of conidiophores and NaOH color reaction | Record the observation results under the microscope in detail and take photos for preservation |
Culture medium preparation and isolation culture technology
Potato Dextrose Selective Medium (PDA)
- Preparation method: Peel the potatoes, boil and filter, add sugar and agar
- Features: Suitable for rapid growth of pathogens, penicillin and streptomycin can be added to inhibit bacteria
Malt Extract Medium (MEA)
- Preparation method: Heat and dissolve the malt extract, add agar to make up the volume
- Features: The culture medium has natural ingredients and is suitable for long-term observation of colony characteristics
Oatmeal Medium (OA)
- Preparation method: Boil and filter the oatmeal, add agar to make up the volume
- Features: economical and practical, suitable for large-scale sample testing
Result determination and record keeping
Samples that meet the characteristics of colony morphology, pathogenicity determination and NaOH color reaction can be identified as tomato Subspora stem rot pathogen. The test results must be properly stored and reviewed regularly.
Implementation suggestions
- Strengthen quarantine awareness and strictly control the import of seeds and plants
- Promote standardized testing procedures and improve the capabilities of grassroots technical personnel
- Establish a regional disease monitoring network and issue early warning information in a timely manner
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