GB/T 36845-2018 in English

Background and Significance of Standard Formulation

Citrus stubborn disease is caused by Spiroplasma citri Saglio et al., which is a quarantine disease that seriously harms citrus plants. This standard is formulated in accordance with GB/T1.1-2009 and aims to standardize the identification method of citrus stubborn disease in inbound plant quarantine and prevent the spread of the disease through international trade.

With the increase in global trade, the frequency of transportation of citrus plants and their products (such as seedlings and fruits) has increased significantly, and the risk of transmission of citrus stubborn disease has also increased. Therefore, it is particularly important to establish a scientific and efficient quarantine and identification method.

Comparison of technical frameworks for detection of stubborn citrus spiroplasma

Implementation suggestions and precautions

1. Sample collection and processing:

• Strictly collect samples in accordance with standard requirements to ensure that the samples are representative.
• Avoid human contamination during sampling, and it is recommended to use aseptic operation procedures.

2. Selection of testing equipment:

• Preferably use high-sensitivity PCR instruments and fluorescent quantitative PCR instruments.
• Ensure that the instrument is accurately calibrated and regularly maintained and updated.

3. Preparation of reagents and culture medium:

• Prepare PCR buffer, dNTPs and culture medium strictly according to standard requirements.
• It is recommended to use high-purity reagents to avoid batch differences affecting the results.