GB/T 39873-2021 in English
VALIDDetermination of quaternary ammonium salt in disinfectant—Liquid chromatography-tandem mass spectrometry
- Issued on:2021-03-09
- Implemented on:2021-10-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
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《GB/T 39873-2021消毒剂中季铵盐的测定 液相色谱-串联质谱法》由TC374（全国质量监管重点产品检验方法标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
Interpretation of the core content of the standard
This standard specifies the analytical method for the determination of six quaternary ammonium compounds in disinfectants using liquid chromatography-tandem mass spectrometry, including: C12-BAC, C14-BAC, C16-BAC, DDAC, DTAB, EDDAB. The detection limit of the method is 0.003g/kg, and the quantitative limit is 0.01g/kg.
Key technical points of the method
| Technical links | Key parameters | Quality control requirements |
|---|---|---|
| Sample pretreatment | Ultrasonic extraction with 0.5% formic acid in methanol, 0.22μm organic membrane filtration | The recovery rate must meet the precision of ≤10% |
| Chromatographic conditions | C18 column (1.7μm), acetonitrile-0.1% formic acid gradient elution | Retention time deviation±2.5% |
| Mass spectrometry conditions | ESI+ mode, MRM monitoring | The ion pair abundance deviation complies with the requirements of Table 3 |
Key points for standard implementation
Typical application scenarios
When a disinfectant manufacturer used this standard to test the C12-BAC content in its products, it was found that the mass spectrometer response value exceeded the linear range. After 10-fold dilution with 0.5% formic acid methanol solution, the accurate result was 2.35g/kg, RSD=3.2%, which met the standard precision requirements.
Technology Evolution Analysis
Compared with traditional titration and HPLC-UV methods, this standard has the following advantages:
- Detection sensitivity is improved by 100 times
- Six quaternary ammonium compounds can be analyzed simultaneously
- Significantly enhanced anti-matrix interference ability
Operation Precautions
- The standard stock solution needs to be stored at -18℃ for no more than 6 months
- When tuning the mass spectrometer, ensure that the collision energy of DDAC (326/186) is 30eV
- The sample centrifuge speed must be ≥8000r/min
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