GB/T 41556-2022 in English

Technical framework of diagnosis and treatment standards

This standard innovatively establishes a three-level diagnosis system: clinical feature determination → etiological testing → nucleic acid/antibody laboratory confirmation, covering the entire process from screening of suspected cases to precise typing diagnosis.

Comparison of key points of detection technology implementation

Innovation in etiological diagnosis technology

The standard clearly defines the criteria for judging dual pathogen mixed infection for the first time: when the specific fragments of 408bp (bovine Babesia) and 646bp (biloba Babesia) are detected at the same time, it is determined to be a mixed infection case (see the figure in Appendix H).

Key parameters for antibody detection

• Serum S/P value ≥ 0.28 determines positive for Bovine Babesia
• S/P ≥ 0.32 determines positive for Double-budded Babesia
• Data collection requires the use of a fully automatic microplate reader

Standard application guide