GB/T 42080.3-2024 in English

This standard, GB/T 42080.3-2024, is issued by the National Standardization Administration Committee and is scheduled to come into effect on April 1, 2025. It specifically addresses the pre-processing steps for DNA isolation in molecular diagnostics involving frozen tissue samples. The third part of this series focuses on methodologies and best practices for extracting high-quality DNA from cryopreserved specimens, ensuring consistency and reliability across various diagnostic applications.

The standard outlines detailed protocols for handling frozen tissues to minimize degradation of nucleic acids during the thawing and processing stages. It also includes recommendations for equipment calibration, reagent selection, and quality control measures that are crucial for maintaining sample integrity throughout the DNA isolation process.

By adhering to these guidelines, laboratories can enhance their diagnostic accuracy in molecular pathology tests, particularly those involving genetic mutations or infectious agents detected through nucleic acid amplification techniques. This standard aims to promote standardized procedures across different institutions and regions, facilitating better comparability of results and advancing research efforts in the field.