GB/T 5009.86-2003 in English

SUPERSEDED

Determination of total ascorbic acid in fruits, vegetables and derived products-Fluorometric method

Issued on:2003-08-11
Implemented on:2004-01-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $220.00
$214.00

Standard No:	GB/T 5009.86-2003
Document status:	SUPERSEDED
Superseded by:	GB 5009.86-2016 National Food Safety Standard-Determination of Ascorbic Acid in Foods
Superseded on:	2017-03-02
Title in English:	Determination of total ascorbic acid in fruits, vegetables and derived products-Fluorometric method
Title in Chinese:	蔬菜、水果及其制品中总抗坏血酸的测定(荧光法和2，4？二硝基苯肼法)
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2003-08-11
Implemented on:	2004-01-01
Superseding:	GB/T 12392-1990 Method for determination of total ascorbic acid in fruits, vegetables and derived products―Fluorometric method and colorimetric method 2.4-dinitrophenylhydrazine
ICS Classification:	67.040-food products in general
Chinese Classification:	C53-Food sanitation
Professional Classification:	GB-National Standard
Related Keywords:	total ascorbic acid products-fluorometric method fluorometric method colorimetric method method
Related Topics:	Secondary Antibody + Fluorescence Fluorescent secondary antibody Fluorescent secondary antibody+ Fluorescence Fluorescent secondary antibody Hydrazine determination method fluorescence response Dinitrophenylhydrazine method Lanthanum nitrate + fluorescence total phosphorus ascorbic acid method Dinitrophenylhydrazine method vegetable and fruit Dinitrophenylhydrazine and ascorbic acid Nitrophenylhydrazine method Dinitrobenzene method Measure fluorescence GB/T5009.86-2003 Phenylhydrazine method Phenylhydrazine method gb5009.86 Nitrophenylhydrazine ascorbic acid Total Ascorbic Acid Content Fluorescence and Determination of total phosphorus ascorbic acid Ascorbic acid in fruit Ascorbic acid determination method total phosphorus ascorbic acid method Primary antibody without fluorescence Secondary antibody with fluorescence Flow cytometry light method Fruit Ascorbic Acid Ascorbic acid determination method gb/t5009.86 GB/T 5009.86 GB/T 5009.86-2003 Analysis of results of determination of ascorbic acid in fruits and vegetables by potassium iodate titration method Method for measuring total ascorbic acid in foods Nitrobenzene spectrophotometry in water Ascorbic acid free radical determination method

《GB/T 5009.86-2003蔬菜、水果及其制品中总抗坏血酸的测定（荧光法和2，4-二硝基苯肼法）》由361（国家卫生健康委员会）归口，主管部门为国家卫生健康委员会。
本标准规定了用荧光法和2,4-二硝基苯肼比色法测定食品中的抗坏血酸。本标准适用于蔬菜、水果及其制品中总抗坏血酸的测定。

1 Scope

This standard specifies determination of total ascorbic acid in the food in fluorometric method and colorimetric method.
This standard is applicable to determination of total ascorbic acid in fruits, vegetables and derived products
Detection limit of this method: 0.022μg/mL for fluorometric method and 0.1μg/mL for colorimetric method. Linear range: 5~20μg/mL for fluorometric method and 1~12μg/mL for colorimetric method.

Contents
Foreword I
1 Scope
2 Principle
3 Reagents
4 Instruments
5 Analysis Procedures
6 Result Calculation
7 Precision
8 Principle
9 Reagents
10 Instruments
11 Analysis Procedures
12 Result Calculation
13 Precision

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