SC/T 9435-2019 in English

VALID

Determination of malachite green in water and sediment from the aquaculture environment- -High performance liquid chromatography

Issued on:2019-08-01
Implemented on:2019-11-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	SC/T 9435-2019
Document status:	VALID
Title in English:	Determination of malachite green in water and sediment from the aquaculture environment- -High performance liquid chromatography
Title in Chinese:	水产养殖环境（水体、底泥）中孔雀石绿的测定高效液相色谱法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2019-08-01
Implemented on:	2019-11-01
Professional Classification:	SC-Aquaculture
Related Topics:	Peacock Water body malachite green Sediment Principle of Liquid Chromatography Malachite Green+ colonizer effect Environmental water body Mass Spectrometry Water Environment Principle of Liquid Chromatography oil in water phase HPLC English Liquid chromatography environment Liquid environment Liquid chromatography environment Liquid environment liquid chromatography for the environment Environmental water body environmental water HPLC liquid + color LC product water Water standard Efficient aquaculture Aquaculture and the Environment HPLC a Moisture measurement environment Underwater mud sc solution sc solution Determination method of high performance liquid chromatography Sediment environmental quality HPLC Environmental sediment Environmentally friendly sediment testing

Introduction

Analysis of the core content of the standard

This standard adopts a high performance liquid chromatograph in conjunction with a fluorescence detector to establish a detection method for malachite green in aquaculture environments (water bodies/sediment), with a quantitative limit of 0.1 μg/L (water bodies) and 1.0 μg/kg (sediment).

Comparison of technical methods

Detection object	Pretreatment method	Chromatographic conditions	Limit of quantification
Aquaculture water	Potassium borohydride reduction + MCX column purification	C18 column, acetonitrile-ammonium acetate mobile phase	0.1 μg/L
Aquaculture sludge	Ultrasonic extraction + alumina purification	Same water conditions	1.0 μg/kg

Key operating points

Sample processing specifications

Water samples must be filtered through a 0.45 μm filter membrane, and potassium borohydride solution must be added to reduce the latent malachite green; sediment samples must be extracted with a dichloromethane-acetonitrile mixture using an ultrasonic cleaner.

Chromatographic parameter setting

Chromatographic column: C18 column (250mm×4.6mm, 5μm)
Mobile phase: acetonitrile-0.125mol/L ammonium acetate buffer (80:20)
Detection wavelength: 265nm (excitation)/360nm (emission)

Standard implementation recommendations

1. The laboratory should regularly verify the recovery rate of the solid phase extraction device, and it is recommended to perform matrix spike experiments for each batch of samples;
2. Note that the standard solution must be prepared and used and stored away from light for no more than 3 months;
3. The water content of the sediment test must be measured simultaneously for result correction.

Technology Evolution Analysis

Compared with the early colorimetric method, this standard has the following advantages:
• Sensitivity is improved by more than 100 times
• Malachite green and its metabolites can be detected simultaneously
• MCX column purification technology is introduced to reduce matrix interference

More samples: JC/T 2453-2018 JGJ 94-2008 JJG 21-2008

Sample only — not a preview of SC/T 9435-2019