SN/T 4724-2016 in English
VALIDDetection and identification of Dendroctonus rufipennis Kirby
- Issued on:2016-12-12
- Implemented on:2017-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$243.00
Introduction
Technical Background of the Standard
SN/T 4724-2016 is an industry standard for entry-exit inspection and quarantine issued by the General Administration of Customs of China. It specifies specific inspection and identification specifications for the Dendroctonus valens, an internationally recognized quarantine pest. This standard integrates traditional morphological identification with modern molecular biology techniques, providing authoritative technical support for port quarantine.
Core identification characteristics
| Characteristic dimensions | Red pine beetle | Comparison with similar species |
|---|---|---|
| Body length range | 5.4-7.3mm | Western pine beetle (3.4-3.7mm) |
| Elephant notches | Arranged regularly in longitudinal stripes | Spruce beetle has a reticular distribution |
| Pronotum | With 4-6 teeth on each side of the base | The number of denticles varies between species. |
Key Technical Requirements
Sample Processing Procedure
The standard stipulates that specimens must be fixed with 75% alcohol and morphological observations must be conducted at 25-28°C and 75-85% humidity. For molecular analysis, the CTAB method is required for DNA extraction and PCR amplification of the mitochondrial COI gene fragment.
Host Plant Range
The primary target species are coniferous species such as Pinus and Picea, with a particular preference for species such as Chinese pine and Armand pine.
Recommendations for the Implementation of the Standard
- Port quarantine should combine trapping and monitoring with wood sampling
- Implement 100% unpacking inspection of softwood packaging materials from epidemic areas in North America
- When suspected individuals are found, microphotography should be conducted to preserve evidence
Analysis of Technological Evolution
Compared with the 2009 version of the old standard, the main upgrades of SN/T 4724-2016 are:
1) Added a real-time fluorescence PCR detection method
2) Improved the identification key table for 27 similar species
3) Supplemented with a high-definition atlas of damage symptoms
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