SN/T 5135-2019 in English
VALIDDetection and identification of Fruticicola fruticum (Müller, 1774)
- Issued on:2019-09-03
- Implemented on:2020-03-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$156.00
| Standard No: | SN/T 5135-2019 |
| Document status: | VALID |
| Title in English: | Detection and identification of Fruticicola fruticum (Müller, 1774) |
| Title in Chinese: | 布氏蜗牛检疫鉴定方法 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2019-09-03 |
| Implemented on: | 2020-03-01 |
| Professional Classification: | SN-Import&Export Inspection |
| Related Keywords: | fruticicola fruticum
quarantine identification measurement indicators optimize coi gene detection system supplement molecular identification molecular detection |
| Related Topics: | snails
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Introduction
Standard Background and Scope of Application
SN/T5135-2019 was issued by the General Administration of Customs to develop a standardized method for quarantine identification of Fruticicola fruticum. This species belongs to the family of Helicidae, class of Gastropoda, class of Mollusca, order of Pulmonaria, and is included in the list of quarantine pests for imported plants in my country.
Core identification features
| Characteristic dimensions | Brown snail | Differences between closely related species |
|---|---|---|
| Shell structure | 5-6 spiral layers, shell height 10-20mm, shell width 13-25mm | 1-2 fewer shell layers, smaller body size |
| Embryonic shell | 1.5-2 layers, smooth and without patterns | Obvious patterns |
| Reproductive system | The length of the penis sheath accounts for 1/7 of the body length | The proportion is significantly different |
Typical case: In 2018, live Brown snails were intercepted from imported wood at Dalian Port. The species was confirmed by the number of shell layers and reproductive anatomical characteristics, and the final determination was made after COI gene sequence comparison (similarity ≥ 95%).
Molecular detection technical specifications
The standard stipulates the use of COI gene barcode for molecular identification:
- Primer sequence: LCO1490/HCO2198
- Amplification conditions: 94℃ pre-denaturation for 5min, 30-35 cycles (94℃50s→45℃30s→72℃50s)
- Expected product: 600-700bp fragment
The similarity with the GenBank reference sequence (AY546264.1) must be ≥95% for confirmation.
Quarantine treatment recommendations
- Immediately soak in 75% alcohol for 24-48 hours when living snails are found
- Improve wooden packaging with methyl bromide 48g/m³, 24h
- Establish snail stress resistance monitoring files
Technology evolution analysis
Compared with the old standard SN/T3067-2011, this version has the following major upgrades:
- New embryo shell microscopic measurement indicators
- Optimize COI gene detection system
- Supplement geographical distribution database
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