SN/T 5184-2020 in English
VALIDQuarantine protocol for avian paratyphoid
- Issued on:2020-12-30
- Implemented on:2021-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
Price(USD)：
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$262.00
| Standard No: | SN/T 5184-2020 |
| Document status: | VALID |
| Title in English: | Quarantine protocol for avian paratyphoid |
| Title in Chinese: | 禽副伤寒检疫技术规范 |
| Language: | English |
| File Format: | Electronic (PDF) |
| Delivery: | Via email within 1~3 business days |
| Issued on: | 2020-12-30 |
| Implemented on: | 2021-07-01 |
| Professional Classification: | SN-Import&Export Inspection |
| Related Topics: | birds
paratyphoid Quarantine technology astm 5184 |
Introduction
Analysis of the core content of the standard
| Diagnostic methods | Key technical points | Judgment criteria | Applicable scenarios |
|---|---|---|---|
| Clinical diagnosis | Observe the symptoms of acute septicemia in chicks/bacterial status in adult chickens | Mortality rate 10-80% | On-site initial screening |
| Pathogen isolation | SC/TTB/RVS enrichment→BS/DHL/XLD plate isolation | Trisaccharide iron reaction characteristics | Laboratory confirmation |
| Glass slide agglutination | Multivalent antigen + whole blood reaction | ≥50% agglutination within 2 minutes | Rapid screening |
| PCR detection | 240bp specific fragment | Electrophoresis band determination | Molecular diagnosis |
Key technical implementation points
1. Pathological sample collection specifications
The standard specifies in detail the processing procedures for 7 types of samples, including cloacal swabs and gall bladder contents:
- Environmental samples require 25g+225mL enrichment solution (SC/TTB)
- Tissues and organs need to be inoculated with non-selective/selective culture media simultaneously
- Egg contents need to be homogenized with 225mL buffered peptone water
2. Serological Identification Process
The standard requires that when AF polyvalent O serum is used for slide agglutination:
- The positive reaction needs to match with O1/O12 and other factor serum
- Self-agglutinating strains need to be confirmed by molecular biology methods
- H antigen identification requires semi-solid agar induction
Standard Evolution and Innovation
Main improvements compared to historical versions:
- Added RVS enrichment solution to improve detection rate
- Introduced Salmonella chromogenic culture medium to shorten detection cycle
- Standardize PCR primer sequences (P1/P2) to ensure specificity
Implementation recommendations
| Application scenarios | Recommended method combination | Precautions |
|---|---|---|
| Rapid screening at ports | Slide agglutination + PCR | Strong/weak positive controls need to be established |
| Laboratory confirmation | Pathogen isolation + serotyping | Biosafety Level II protection |
| Epidemiological investigation | PFGE molecular typing | SN/T 5364 method needs to be supplemented |
Sample only — not a preview of SN/T 5184-2020
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