SN/T 5281-2020 in English

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Standard No:	SN/T 5281-2020
Document status:	VALID
Title in English:	Quarantine protocol for avian Tembusu virus infection
Title in Chinese:	禽坦布苏病毒病检疫技术规范
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-12-30
Implemented on:	2021-07-01
Professional Classification:	SN-Import&Export Inspection
Related Topics:	Virus super virus viral disease m2 virus virus technology shared virus birds virus+ Hantavirus plague iso5281 Sue In addition to virus detection virus standard How to detect virus secretory virus upstream primer virus device Epigastric virus virus detector Abnormal disease Virus virus test q7 virus m2 Virus Prion transient virus upstream primer Device virus t-virus prions female virus Prion disease ancient virus Detection of r5 virus x4 virus Tank Quarantine technology Virus system? Virus system? Virus cold virus prionlow temperature component virus Bali virus broad-spectrum virus Prion Quarantine t virus su a plague virus antivirus portable viral protein virus artificial virus-like simply P virus Comparison of viral prions Viralin discontinued Preparation of depc water ev70 virus The difference between viruses, viroids, and prions retro virus The difference between prions and viruses Inspection and quarantine of quarantine pests and diseases Virus recovery methods Drug testing technology Border disease quarantine technology Cup-like virus detection technology

Introduction

Analysis of standard technical framework

Technical modules	Core parameters	Innovation points
Clinical diagnosis	Sudden rise in body temperature, decrease in egg production rate ≥30%, follicular bleeding	Establishment of a list of specific symptoms for breeding poultry for the first time
RT-PCR detection	NS5 gene 469bp fragment, 54℃ annealing temperature	Degenerate base Y=C/T primer design
Result determination	Sequence homology ≥95%	Introduction of sequencing verification step

The tissue homogenizer used in the standard must meet the centrifugation requirement of 8,000r/min, and the RNA extraction step is clear:

Trizol method organic phase separation requires 4℃ 12,000r/min centrifugation for 15min
The isopropanol precipitation step requires -20℃ pre-cooling

In 2022, a laying hen farm in Fujian experienced a 42% drop in egg production. Through the detection of this standard, it was found that:

Ovarian sample electrophoresis showed a 469bp band
Sequencing results had a homology of 97.3% with the reference sequence
Implement isolation measures after diagnosis to prevent the spread of the epidemic

Compared with the 2015 Ministry of Agriculture industry standard, this version has the following major upgrades:

Added standardized use requirements for UV gel imaging system
Specified that DEPC water-treated centrifuge tubes must be autoclaved at 121℃ for 30min
Supplemented the equivalence recognition clauses for commercial kits

More samples: QC/T 942-2021 JJG 21-2008 JC/T 2453-2018

Sample only — not a preview of SN/T 5281-2020

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