SN/T 5335-2020 in English

Analysis of the core content of the standard

In view of the particularity of African swine fever virus detection, this standard establishes a biosafety protection system from three dimensions: laboratory hierarchical management, sample full process control and disinfection and sterilization specifications:

Key technical points

1. Sample handling specifications

All operations in the biological safety cabinet must comply with the following:

• Tissue samples must be made into a 10% homogenate and inactivated at 60℃ for 30 minutes
• Anticoagulated blood must be directly packaged and inactivated, and non-anticoagulated blood must be allowed to stand for 12-24 hours to separate serum
• Tick samples must be pre-cooled and ground, and centrifuged at 1000g to obtain the supernatant

2. Disinfection and sterilization requirements

It is recommended to use the following disinfectants for 30 minutes:

Suggestions for the implementation of the standard

1. Focus of staff training

The following skills training needs to be strengthened:

1. Sample spill emergency disposal process (according to Clause 7.1)
2. Autoclave operation verification (maintaining 121℃ for 30 minutes)
3. Level 3 packaging and transportation specifications (Announcement No. 503 of the Ministry of Agriculture)

2. Key points of quality control

The following quality control records should be established:

• Daily biosafety cabinet wind speed monitoring records
• Autoclave effect biological indicator test records
• Positive sample transfer cold chain temperature records

Technology Evolution Analysis

Compared with the earlier version, the main progress of this standard is reflected in:

• Added Tick Sample Processing Specifications
• Clarified the laboratory grade requirements for different detection methods
• Refined the parameters for disinfectant selection and action time