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standard establishes key technical indicators grade length special grade ≥70 ≥2.0 ≥0.10 microscopy+thin layer+dna barcode first three-level quality control model grade acid products deposited metal scopethis standard food plastic turnover boxes
T/CACM 1329.2-2019 in English

T/CACM 1329.2-2019 in English

VALID

Quality grade standard of ZizhouHuangqi

  • Issued on:2019-11-28
  • Implemented on:2020-01-01
  • File Format:PDF
  • Delivery:Via email within 1~3 business days
Price(USD): $280.00
$272.00
Standard No: T/CACM 1329.2-2019
Document status: VALID
Title in English: Quality grade standard of ZizhouHuangqi
Title in Chinese: 子洲黄芪药材质量等级标准
Language: English
File Format: Electronic (PDF)
Delivery: Via email within 1~3 business days
Issued on: 2019-11-28
Implemented on: 2020-01-01
Professional Classification: T/-Social Organization Standard
Related Keywords: standard establishes
key technical indicators grade length
special grade ≥70 ≥2.0 ≥0.10 microscopy+thin layer+dna barcode first
three-level quality control model
grade
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Introduction

Analysis of the core content of the standard

This standard establishes for the first time a standardized grading system for Zizhou Astragalus simulated wild Mongolian Astragalus, and through the three-level quality control model of trait-ingredient-molecule, establishes an evaluation framework for 6 commodity grades.


Comparison of key technical indicators

0.04
Grade Length(cm) Upper diameter(cm) Astraloside IV(%) Identification method
Special grade ≥70 ≥2.0 ≥0.10 Microscopy+thin layer+DNA barcode
First grade ≥60 ≥1.5 ≥0.10
Second grade
≥50 ≥1.3 ≥0.10
Third Class ≥40 ≥1.0 ≥0.08
Fourth Class ≥30 ≥0.8 ≥0.06
Fifth Class - ≥0.6 ≥0.04

Application of Innovative Technologies

DNA Barcode Identification

The psbA-trnH sequence was used as a molecular marker to ensure the accuracy of the gene through the PCR amplification process specified in Appendix B. The dominant haplotype sequence had characteristic insertions/deletions at sites 93, 202-206, etc.

Dual indicators for ingredient control

  • Astragaloside IV: HPLC-ELSD method, mobile phase is acetonitrile-water (32:68)
  • Varisol glucoside: HPLC-UV method, gradient elution procedure see standard 7.6.2.2

Implementation key recommendations

  1. The harvesting period must be ≥5 years to ensure the accumulation of active ingredients
  2. Pay special attention to the limits of lead (≤5mg/kg) and arsenic (≤2mg/kg) for heavy metal detection
  3. The key to evaluating properties is to observe the cross-section characteristics of "Jinjing Yulan" and the morphology of "straight as an arrow shaft"
  4. Storage moisture control ≤10.0% to prevent mildew

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