T/CACM 1329.2-2019 in English
VALIDQuality grade standard of ZizhouHuangqi
- Issued on:2019-11-28
- Implemented on:2020-01-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$272.00
Introduction
Analysis of the core content of the standard
This standard establishes for the first time a standardized grading system for Zizhou Astragalus simulated wild Mongolian Astragalus, and through the three-level quality control model of trait-ingredient-molecule, establishes an evaluation framework for 6 commodity grades.
Comparison of key technical indicators
| Grade | Length(cm) | Upper diameter(cm) | Astraloside IV(%) | Identification method |
|---|---|---|---|---|
| Special grade | ≥70 | ≥2.0 | ≥0.10 | Microscopy+thin layer+DNA barcode |
| First grade | ≥60 | ≥1.5 | ≥0.10 | |
| Second grade | 0.04||||
| ≥50 | ≥1.3 | ≥0.10 | ||
| Third Class | ≥40 | ≥1.0 | ≥0.08 | |
| Fourth Class | ≥30 | ≥0.8 | ≥0.06 | |
| Fifth Class | - | ≥0.6 | ≥0.04 |
Application of Innovative Technologies
DNA Barcode Identification
The psbA-trnH sequence was used as a molecular marker to ensure the accuracy of the gene through the PCR amplification process specified in Appendix B. The dominant haplotype sequence had characteristic insertions/deletions at sites 93, 202-206, etc.
Dual indicators for ingredient control
- Astragaloside IV: HPLC-ELSD method, mobile phase is acetonitrile-water (32:68)
- Varisol glucoside: HPLC-UV method, gradient elution procedure see standard 7.6.2.2
Implementation key recommendations
- The harvesting period must be ≥5 years to ensure the accumulation of active ingredients
- Pay special attention to the limits of lead (≤5mg/kg) and arsenic (≤2mg/kg) for heavy metal detection
- The key to evaluating properties is to observe the cross-section characteristics of "Jinjing Yulan" and the morphology of "straight as an arrow shaft"
- Storage moisture control ≤10.0% to prevent mildew

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