WS/T 191-2017 in English

VALID

Chancroid Diagnosis

Issued on:2017-07-24
Implemented on:2018-02-01
File Format:PDF
Delivery:Via email within 1~3 business days

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$339.00

Standard No:	WS/T 191-2017
Document status:	VALID
Title in English:	Chancroid Diagnosis
Title in Chinese:	软下疳诊断
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-07-24
Implemented on:	2018-02-01
Chinese Classification:	C59-
Professional Classification:	WS-Hygiene

Introduction

Background and Significance of the Standard Revision

Major changes in the 2017 standard compared to the 1999 version include: changing the standard's nature from mandatory to recommended, adding new term definitions, deleting sections on treatment and management, and updating laboratory testing methods. This reflects the medical community's deepening understanding of Haemophilus ducreyi and a greater focus on diagnostic accuracy.

Analysis of core diagnostic elements

Diagnostic basis	Clinical features	Laboratory requirements
Suspected cases	Painful ulcers + satellite lesions	Exclude syphilis/HSV infection
Confirmed cases	Typical clinical manifestations	Positive bacterial culture

Key points of laboratory testing techniques

Gram stain microscopy

Standard requirements for detection class=instrument>Gram-negative short rods (1.6-2μm), but it is emphasized that the false positive rate of this method is as high as 30%, and it needs to be combined with culture results.

Key points of bacterial culture

Culture medium selection: Modified Thayer-Martin or chocolate agar (containing 3mg/L vancomycin)
Culture conditions: 33-35℃, 5-10% CO₂ environment, 48-72 hours of observation
Colony characteristics: 1-2mm translucent colonies, positive in the push test

Comparative analysis of the new and old standards

Project	WS 191-1999	WS/T 191-2017
Nature of the standard	Mandatory	Recommendation
Appendix content	Includes treatment plan	Added culture medium preparation specifications
Detection method	Fluorescent antibody staining method	Added alkaline phosphatase test

Clinical implementation recommendations

Syphilis, HSV, and HIV should be tested simultaneously in patients with genital ulcers
Necrotic tissue on the ulcer surface should be removed and samples should be taken from the base
PCR testing is recommended for highly suspected cases with negative culture
Prolonged observation period is required for patients with co-HIV infection

More samples: NB/T 47013.2-2015 DL/T 368-2010 GB 50021-2001(2009)

Sample only — not a preview of WS/T 191-2017