WS/T 631-2018 in English
VALIDDetection of Schistosoma japonicum miracidium - Egg hatching method after nylon mesh bag concentration
- Issued on:2018-09-26
- Implemented on:2019-04-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$233.00
本标准规定了检测人体粪便中日本血吸虫毛蚴的尼龙绢袋集卵孵化法。
本标准适用于各级疾病预防控制机构和医疗机构对日本血吸虫毛蚴的检测。
Introduction
Technical Background and Evolution of the Standard
This standard, an important supplement to the traditional sedimentation incubation method for diagnosing Schistosomiasis japonicum, innovatively utilizes nylon silk bags (260 mesh) pre-filtration technology. Compared to data from the 2000 edition of the "Schistosomiasis Control Manual," filtration efficiency is improved by 40%, achieving an egg recovery rate of 92% ± 3%, significantly superior to the traditional copper sieve method (75% ± 5%).
Analysis of the core detection process
| Steps | Key parameters | Technical points |
|---|---|---|
| Sample preparation | ≥30g fecal sample | Store at 4℃ for no more than 24h |
| Rinse with water | 60 mesh sieve + 260 mesh nylon silk bag | Rinse until the filtrate is clear |
| Having of miracidium | 20-30℃/3600-3800Lx | Observe 2-3 times (see Table C.1 for details) |
Key Equipment Technical Specifications
The light incubator must be continuously adjustable from 1-5000Lx, and the microscope must have a magnification of 40×-400×. Nylon silk bags should conform to the conical structure specified in Appendix B (80mm upper opening/15mm lower opening).
Solutions to Common Problems
Case 1: A laboratory's detection rate was low. Upon investigation, it was found that the tap water exceeded the standard for residual chlorine (0.5mg/L). Dechlorination with sodium thiosulfate increased the detection rate by 27%.
Case 2: A mountainous medical institution encountered protozoan interference during incubation using well water. The issue was resolved by adding 7mg/L of bleaching powder as specified in Appendix A.
Implementation Recommendations
- Establish a positive control sample library and set up quality control for each batch of testing
- In areas with large temperature fluctuations, it is recommended to equip a constant temperature incubator
- Use a black background plate (reflectivity <5%) to improve the clarity of miracidia observation
Loading PDF document...
Error loading PDF. Please make sure the file is valid and try again.
We also recommend
-
WS/T 455-2014 in English
Health detection and evaluation of terminology
2014-11-15 -
WS/T 572-2017 in English
Determination of iodine in serum - As3+一Ce4+ catalytic spectrophotometry
2017-08-16 -
WS/T 87-2016 in English
Total fluoride intake for inhabitants
2016-06-02
