GB/T 13087-2020 in English
VALIDDetermination of isothiocyanates in feeds
- Issued on:2020-07-21
- Implemented on:2021-02-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$156.00
Introduction
Key points of standard revision
| Technical parameters | GB/T13087-1991 | GB/T13087-2020 | Improvement significance |
|---|---|---|---|
| Detection method | Argentometric method + gas chromatography | Gas chromatography (delete argentometric method) | Eliminate the risk of heavy metal pollution |
| Detection limit | Undefined | 2mg/kg | Quantitative detection capability |
| Dosage of mustardase | 1.0g/sample | 0.5g/sample | Reducing detection costs |
Key technological breakthroughs
The new standard adopts a gas chromatograph in combination with a hydrogen flame detector (FID), and optimizes the chromatographic conditions: FFAP capillary column (30m×0.32mm×0.25μm), programmed temperature (100℃ for 10min→10℃/min→200℃ for 2min), which significantly improves the separation of propenyl, butenyl and pentenyl isothiocyanates.
Analysis of implementation points
Key points for the preparation of mustard enzyme
According to GB/T5520, mustard seeds with a germination rate of >85% were screened, defatted with petroleum ether 10 times to reduce the fat content to <2%, crushed and passed through a 0.28mm sieve, and stored at -18°C with a shelf life of 6 months. Notes during preparation:
- Ambient temperature ≤30℃
- Crush in batches to prevent overheating
- Activity verification needs to be performed for each batch
Test process optimization
- Sample preparation: 2.0g sample + 0.5g mustard enzyme + 5mL pH7.0 buffer + 10mL internal standard solution
- 200 times/min oscillation extraction for 2h
- 5000r/min centrifugation for 10min and then filtration
- Gas chromatography analysis (injection volume 1μL, split ratio 5:1)
Standard implementation recommendations
Laboratories should focus on:
- The mixed standard solution of isothiocyanates needs to be stored at -18℃ and has a shelf life of 3 months
- Regularly verify the separation efficiency of the chromatographic column (relative retention time deviation ≤±2.5%)
- Precision control: RSD≤15% when ≤100mg/kg, RSD≤10% when >100mg/kg
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