GB/T 15670.19-2017 in English

VALID

Toxicological test methods for pesticides registration―Part 19:In vitro mammalian cells chromosome aberration test

Issued on:2017-07-12
Implemented on:2018-02-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $100.00
$97.00

Standard No:	GB/T 15670.19-2017
Document status:	VALID
Title in English:	Toxicological test methods for pesticides registration―Part 19:In vitro mammalian cells chromosome aberration test
Title in Chinese:	农药登记毒理学试验方法第19部分：体外哺乳动物细胞染色体畸变试验
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-07-12
Implemented on:	2018-02-01
ICS Classification:	65.100-Pesticides and other agrochemicals
Chinese Classification:	B17-Pesticide management and application method
Professional Classification:	GB-National Standard
Related Keywords:	vitro mammalian cell chromosome aberration test chromosome aberration test introduction toxicological test methods chromosome aberration rates positive reaction rate test methods use
Related Topics:	chromosome Chromosomal aberration cell chromosomal aberration cell staining cell staining In vitro mammalian cell micronucleus test method mammalian cells Chromosomal aberration assay in mammalian cells in vitro time cell chromosome test Toxicology+ mammalian exosomes mammalian cells distortion chromosome method mammalian chromosome Statistical significance chromosomal cells Toxicology test pesticide cell Toxicology test Pesticide Registration Toxicology Test Pesticide Toxicology Test Methods Pesticide Registration Toxicology Test blocking agent In vitro/be Toxicology test methods for pesticide registration "chromosome Chromosome+ Pesticide Registration Toxicology GB/T 15670.19 GB/T 15670.19-2017 Chromosomal aberrations in mammalian cells in vitro Pharmacological cell experiments Materials live and dead cell staining methods Cell crystal violet staining method Cell methodology validation In vitro mammalian cell chromosome aberration test method Intracellular drug concentration verification method Methods for extracellular electrophysiological recording How to stain cells with trypan blue Animal poisoning routes and methods Methods for testing chromosomes in animal samples Advanced methodology of transfection cell method Cellular iron staining methods What are the methods for preparing animal chromosomes? Cell transfection procedures Staining method cells Common cell staining methods Nucleus staining method Cell invasion assay staining method Cell chromosome separation method Cell chromosome extraction method The most common cytochemical staining methods Large-scale culture method of mammalian cells Cell transfection method There are several cell staining methods. Mammalian cell transfection methods

《GB/T 15670.19-2017农药登记毒理学试验方法第19部分：体外哺乳动物细胞染色体畸变试验》由326（农业农村部）归口，主管部门为农业农村部。

Introduction

1. Analysis of the background of standard formulation and technological evolution

GB/T 15670.19-2017 "Toxicological test methods for pesticide registration Part 19: In vitro mammalian cell chromosome aberration test" is a standard formulated to evaluate the mutagenicity of pesticides. With the increase in the use of pesticides, their potential genetic toxicity risks to humans and the environment are becoming increasingly prominent. By detecting chromosome aberrations through in vitro tests, pesticides with genetic toxicity can be effectively identified, providing a scientific basis for registration management.

2. Comparison of standard frameworks

Standard dimensions	GB/T 15670.19-2017	Internationally accepted standards	Evaluation indicators
Purpose of the test	To detect whether pesticides cause chromosome aberrations in mammalian cells in vitro	OECD Guideline 476	Positive reaction rate
Test methods	Use of metaphase blockers and microscopic observation	NMPA/EMA requirements	Aberration type analysis
Dose setting	At least 3 analytical concentrations, the highest concentration must significantly reduce cytotoxicity	ICH S2(R1)	Statistical significance

3. Implementation suggestions

Experimental design suggestions:In actual operation, cell lines with high stability and strong proliferation ability (such as CHO or CHL) should be given priority. The selection of positive controls should be based on the physical and chemical properties of the test, and negative controls are used to exclude the influence of solvents.

Data processing suggestions:During statistical analysis, it is recommended to use the χ2 test to evaluate significant changes in chromosome aberration rates. If positive results of dose dependence and repeatability are observed, judgments should be made based on biological significance and statistical evidence.

4. Technical details and precautions

Cell culture conditions: Eagle's minimum essential medium with a serum concentration of 10% is recommended. The incubator needs to maintain a 5% CO2 environment.
Metaphase blocker usage: Colchicine concentration is 1μg/mL, and the treatment time is 4 hours.
Chromosome analysis: At least 200 metaphase cells were observed in each treatment group. Aberration types include deletion, breakage and recombination.

More samples: YD/T 3934-2021 JJG 21-2008 NB/T 47013.2-2015

Sample only — not a preview of GB/T 15670.19-2017

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