GB/T 16551-2020 in English

VALID

Diagnostic techniques for classical swine fever

Issued on:2020-12-14
Implemented on:2020-12-14
File Format:PDF
Delivery:Via email within 5 business days

Price(USD)： $310.00
$301.00

Standard No:	GB/T 16551-2020
Document status:	VALID
Title in English:	Diagnostic techniques for classical swine fever
Title in Chinese:	猪瘟诊断技术
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 5 business days
Issued on:	2020-12-14
Implemented on:	2020-12-14
Superseding:	GB/T 16551-2008 Diagnostic techniques for classical swine fever(hog cholera)
ICS Classification:	11.220-Veterinary medicine
Chinese Classification:	B41-Animal quarantine, veterinary and epidemic prevention
Professional Classification:	GB-National Standard
Related Topics:	swine fever diagnostic technology semi-quantitative diagnosis ncu plague Condition Diagnosis Technology Profiler technology Diagnostic antibody diagnostic technology diagnostic regulations Measures swine fever diagnosis In Vitro Diagnostics for Biotechnology Research swine fever meat Plague must Plague Plague Diagnosis Auxiliary Diagnosis male plague swine fever pork Diagnosis Supplementary Diagnosis Chain plague chain + plague Classification of diagnostic techniques African swine fever diagnosis swine fever molecular diagnostics Early Diagnosis Technology Plague Plague malaria diagnostic ion plague Automatic Diagnosis Technology Endemic swine fever technical diagnostic assessment swine fever, pork Plague also Diagnosis Routine Diagnosis Swine Fever USA Molecular diagnostic cutting-edge technology Technical diagnostic method Diagnostic Technology Testing swine plague pork p4 Molecular Diagnostics plague GBT16551 GB/T 16551-2008 GB/T 16551-2020 How is swine fever virus diagnosed diagnostic meaning Hitachi Diagnostics Academic Department crispr molecular diagnostic technology swine fever place

《GB/T 16551-2020猪瘟诊断技术》由TC181（全国动物卫生标准化技术委员会）归口，主管部门为农业农村部。

Introduction

Background of Standard Revision and Technological Evolution

GB/T 16551-2020 is the core technical standard for the diagnosis of classical swine fever in my country. It mainly targets the upgrade of three major technical requirements that emerged after the implementation of the 2008 version:

Technical Dimensions	2008 Version	2020 Version	Improvement Significance
Detection Sensitivity	RT-PCR	RT-nPCR/Real-time Fluorescence RT-PCR	Detection Limit Reduced by 10-100 Times
Antibody Detection Method	2 ELISAs	Blocking ELISA+Indirect ELISA+Chemiluminescence	Covering immune assessment and wild virus identification
Sample processing specifications	Not clear	Added Chapter 5 Standardized Process	Reduce the risk of false negatives

Core diagnostic technology system

I. Etiology detection method

Immunofluorescent antibody test (FAT) and Immunoperoxidase test (IPT) are used as the gold standard for rapid screening:

Sensitivity comparison: FAT has a detection limit of 10² TCID₅₀/g for tonsil samples, and IPT can preserve slices for a long time
Operation points: The sample needs to be fixed with acetone at -20℃, FAT needs to be observed in the dark, and the IPT color development time is controlled within 5-10 minutes

II. Antibody detection technology

The new standard constructs a three-level antibody detection system:

Method

Detection target

Applicable scenario

Threshold value

Blocking ELISA

E2 protein antibody

Breed pig introduction detection

Inhibition rate ≥40%

Chemiluminescence method

Neutralizing antibody

Immune effect evaluation

≥20

Required organs: spleen, kidney, distal ileum
Auxiliary samples: submandibular lymph nodes, pancreas

Laboratory biosafety

All operations must be carried out in a BSL-2 environment. 2% The NaOH solution was allowed to act for more than 30 minutes.

Vaccine immune interference was excluded

For pigs immunized within 50 days, genotyping (Appendix B of the Standard) was required by RT-nPCR to distinguish between wild virus and vaccine virus (C strain).

More samples: NB/T 47013.2-2015 DL/T 368-2010 GB 50021-2001(2009)

Sample only — not a preview of GB/T 16551-2020

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