GB/T 18204.3-2025 in English
VALIDExamination methods for public places—Part 3：Airborne microorganism indicators
- Issued on:2025-05-30
- Implemented on:2025-12-01
- File Format:PDF
- Delivery:Via email within 5 business days
$422.00
《GB/T 18204.3-2025公共场所卫生检验方法 第3部分：空气微生物指标》由628（国家疾控局）归口，主管部门为国家疾控局。
Introduction
Core changes in the standard revision
| Technical elements | 2013 edition | New content in the 2025 edition |
|---|---|---|
| Sample storage and transportation | Not clearly specified | New 4°C storage/4 hour time limit requirement |
| Quality control | Basic requirements | Add 7 quality control items such as culture medium sterility inspection and sampler calibration |
| Legionella pneumophila detection | Culture method only | Newly added fluorescent PCR rapid detection method (Chapter 7.2) |
Detailed explanation of key detection methods
1. Technical specifications of the impact method
Use six-stage sieve hole impact microbial sampler, the standard stipulates:
- Sampling flow rate: 28.3 L/min (regular calibration is required)
- Culture conditions: bacteria 36±1℃/48h, fungi 28±1℃/3-5d
- Calculation formula:
$$C=\frac{\sum_{i=1}^{6}N_i×1000}{v×t}$$
2. Key points of the natural sedimentation method
Typical application scenarios: Small and medium-sized beauty salons, hair salons and other places with a business area of less than 50m² should set up 3 sampling points according to standard requirements and send the samples for inspection immediately after exposure for 5 minutes.
Evolution of Microbial Detection Technology
Main innovations of the 2025 version:
- Legionella pneumophila detection has added a fluorescent PCR method, and the detection cycle has been shortened from 10 days to 4 hours
- Five biochemical identification steps such as Gram staining and microscopy have been added to β-hemolytic Streptococcus
- All indicators have added sample transportation and storage specifications
Implementation Suggestions
Laboratory Construction Requirements
Configuration required for detecting Legionella pneumophila:
- CO2 incubator (5% concentration)
- Biosafety Level 2 laboratory
- Ultraviolet lamp (360±2nm)
Precautions for on-site sampling
| Environmental factors | Control requirements |
|---|---|
| Door and window status | Close for 15-30 minutes (shopping malls, etc. need to remain in their original state) |
| Crowd density | Record the real-time number of people |
| Temperature and humidity | Detailed records are required |
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