GB/T 19557.9-2017 in English

VALID

Guidelines for the conduct of tests for distinctness，uniformity and stability- Mustard(Brassica juncea(L.)Czern. et Coss)

Issued on:2017-11-01
Implemented on:2018-05-01
File Format:PDF
Delivery:Via email within 5 business days

Price(USD)： $630.00
$612.00

Standard No:	GB/T 19557.9-2017
Document status:	VALID
Title in English:	Guidelines for the conduct of tests for distinctness，uniformity and stability- Mustard(Brassica juncea(L.)Czern. et Coss)
Title in Chinese:	植物品种特异性、一致性和稳定性测试指南芥菜
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 5 business days
Issued on:	2017-11-01
Implemented on:	2018-05-01
ICS Classification:	65.020.20-Plant growing
Chinese Classification:	B05-Agriculture and forestry technology
Professional Classification:	GB-National Standard
Related Keywords:	stem mustard stability- mustard leaf mustard root mustard mustard
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《GB/T 19557.9-2017植物品种特异性、一致性和稳定性测试指南芥菜》由TC277（全国植物新品种测试标准化技术委员会）归口，主管部门为农业农村部。

Introduction

Interpretation of the core content of the standard

This standard specifies the technical specifications for the testing of mustard (Brassica juncea) variety specificity (Distinctness), uniformity (Uniformity) and stability (DUS), and is applicable to 16 varieties including leaf mustard, stem mustard, root mustard and stalk mustard and their hybrid varieties.

Key Technical Requirements

Propagation Material Requirements

Material Type	Seed Amount	Clarity≥	Germination Rate≥	Moisture Content≤
Parental Line	100g	98%	85%	8%
Hybrid	100g	98%	90%	8%
Conventional type	100g	98%	90%	8%

Note:The detection method must be carried out in accordance with GB/T 3543.3, GB/T 3543.4 and GB/T 3543.6.

Test Method Specifications

Field Experiment Design

Period:At least 2 independent growth cycles
Plot Setting:The variety to be tested is planted adjacent to similar varieties, with ≥100 plants per plot, 2 replications
Previous Crop Restriction:Cruciferous crop rotation is prohibited

Trait Observation System

Observation Type	Code	Definition	Sample Size
Population Measurement	MG	Measure a batch of plant organs to obtain population records	Entire plot
Individual measurement	MS	Measure plant organs one by one to obtain individual records	20 plants
Group visual inspection	VG	Visually inspect a batch of plant organs to obtain group records	Entire plot
Individual visual inspection	VS	Visually inspect plant organs one by one to obtain individual records	20 plants

DUS judgment criteria

Specificity judgment

The variety to be tested must have significant and reproducible differences from all known varieties in at least one trait.

Consistency threshold

Variety type	Population standard	Acceptance probability	200 samples allow for heteromorphic strains
Conventional varieties/hybrids	4%	95%	≤13 strains
Maintainer line/paternal line	2%	95%	≤7 strains
Sterile line	1%	95%	≤5 strains

Stability presumption

The stability of trait expression is verified by planting the next generation of seeds, and hybrids can be indirectly determined by the stability of their parents.

Key grouping traits

Cotyledon anthocyanin II coloration (trait 1)
Plant branching (trait 3)
Midrib tubercles (trait 24)
Petiole length/leaf length ratio (trait 27)
Leaf clasping characteristics (trait 31)
Stem enlargement characteristics (trait 37)
Enlarged stem tubercles (trait 42, only for stem enlargement varieties)
Axillary bud enlargement (trait 47, only for stem enlargement varieties)
Root enlargement (trait 53)
Bud appearance period (trait 60)

Implementation suggestions

Testing precautions

Trait observations must be carried out strictly in accordance with the growth stages specified in Appendix A (Table B.1)
Quality traits (QL) and quantitative traits (QN) require different statistical analysis methods
For hybrids, the DUS characteristics of parental materials should be tested simultaneously

Fill in technical questionnaire

The applicant must provide the following in detail as required by Appendix C

Variety breeding process and pedigree information
Trait difference comparison table with similar varieties
Description of suitable planting areas for the variety
Representative plant photos (observed traits must be marked)

More samples: JC/T 2453-2018 JJG 21-2008 YD/T 3934-2021

Sample only — not a preview of GB/T 19557.9-2017

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