GB/T 20189-2025 in English
VALIDDetermination of β-receptor agonists in feeds—Liquid chromatography-tandem mass spectrometry
- Issued on:2025-06-30
- Implemented on:2026-01-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
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《GB/T 20189-2025饲料中β-受体激动剂的测定 液相色谱-串联质谱法》由TC76（全国饲料工业标准化技术委员会）归口，主管部门为国家标准委。
Introduction
Core Technology Innovation of Standards
| Technical Parameters | GB/T 20189-2006 | GB/T 22147-2008 | GB/T 20189-2025 | |||
|---|---|---|---|---|---|---|
| Test Objects | Ractopamine | 3 β-receptor agonists | Limit of Quantitation (μg/kg) | 50 | 10 | 10-50 |
| Pretreatment method | Hydrochloric acid extraction + SPE | Methanol extraction + SPE | Hydrochloric acid-methanol extraction + dual-mode cleanup |
Analysis of key operation points
New extraction system: Using a hydrochloric acid-methanol (4:1) mixed solution, the extraction efficiency is increased by 30% compared with the old standard, especially for hydrophobic compounds such as mabuterol, the recovery rate reaches 92-105%.
Dual-channel purification scheme:For different feed types:
1) Compound feed uses a mixed cation exchange column (MCX)
2) Premix is directly purified using a double filter membrane to reduce matrix interference
Mass spectrometry parameter optimization strategy
Dynamic MRM technology is used to detect 21 compounds in segments according to retention time. Key parameters:
- Sheath gas temperature: 400℃ (50℃ higher than conventional)
- Collision energy: 12-40eV gradient optimization
- Dwell time: 20-50ms/channel
Implementation suggestions
- Standard Material Management: It is recommended to purchase certified standard solutions. Easily degradable compounds such as clenbuterol should be stored at -80°C.
- Quality Control Points: Each batch of samples should be spiked with an isotope internal standard such as clenbuterol-D9. The recovery rate should be controlled at 85-110%.
- Method Validation: Matrix effects should be evaluated for each of the 21 compounds. The standard addition method is recommended for correction.
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