GB/T 24369.3-2017 in English
VALIDCharacterization of gold nanorods. Part 3: Measurement method of surface charge density
- Issued on:2017-07-31
- Implemented on:2018-02-01
- File Format:PDF
- Delivery:Within 1 day
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《GB/T 24369.3-2017金纳米棒表征 第3部分:表面电荷密度测量方法》由TC279(全国纳米技术标准化技术委员会)归口,主管部门为中国科学院。
Introduction
1. Background and significance of standard formulation
Gold nanorods are an important nanomaterial with broad application prospects in the fields of biological detection and disease diagnosis. In order to accurately evaluate the stability of its colloidal solution and its interaction with other substances, GB/T 24369 "Characterization of Gold Nanorods" is divided into three parts. This part (Part 3) focuses on the measurement method of surface charge density.
2. Principle of surface charge density measurement
Surface charge density is an important parameter calculated indirectly through zeta potential. According to the classical double layer model (Gouy-Chapman-Stern model), the surface potential of the particle and the electrolyte ion strength jointly determine the surface charge density.
| Parameter name | Definition | Calculation formula |
|---|---|---|
| Ionic strength (I) | A comprehensive measure of the concentration of all ions in the solution | $$ I = \frac{\sum c_{i}Z_{i}^{2}}{2} $$ |
| Debye length (κ⁻¹) | Thickness of the double layer | $$ κ^{-1} = \frac{0.304}{\sqrt{I}} $$ |
3. Measurement instruments and methods
zeta potential meter is the core instrument for measuring surface charge density. The electrophoretic light scattering method can accurately determine the zeta potential value of gold nanorods, and the surface charge density can be calculated by combining the formula.
4. Implementation suggestions and precautions
- Sample pretreatment: Ensure that the concentration of the test sample is within the measurement range of the instrument and use the same medium for dilution.
- Measurement conditions: Parameters such as temperature and equilibrium time must be strictly controlled. It is recommended to refer to the experimental conditions in Appendix A and B.
- Data processing: For small zeta potential values (<50mV), it is preferred to use the simplified formula (Formula 6) for calculation.

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