GB/T 30399-2013 in English

《GB/T 30399-2013皮革和毛皮 化学试验 致癌染料的测定》由TC252（全国皮革工业标准化技术委员会）归口，主管部门为中国轻工业联合会。
本标准规定了染色皮革、毛皮产品中9种致癌染料及能裂解释放有害芳香胺的分散黄23、分散橙149染料的测定方法。
本标准适用于染色皮革、毛皮产品。

1 Scope

This standard specifies the determination methods of 9 carcinogenic dyestuffs in dyed leather and fur products as well as disperse yellow 23 and disperse orange 149 which can release harmful aromatic amines through splitting decomposition.

This standard is applicable to dyed leather and fur products.

2 Normative references

The following documents for the application of this document are essential. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.

GB/T 6682 Water for analytical laboratory use—Specification and test methods
QB/T 1267 Finished fur products—Sample piece—Position and marking
QB/T 1272 Finished fur products—Preparation of chemical analysis sample and general rules for chemical analysis
QB/T 2706 Leather—Chemical physical and mechanical and fastness tests—Sampling location
QB/T 2716 Leather—Preparation of chemical test samples

3 Principle

The sample is extracted by ultrasonic wave of methanol and filtered by filter membrane. The sample is determined and confirmed by high-performance liquid chromatography with diode array detector (HPLC-DAD) or high-performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS), and quantified by external standard method.

4 Reagents

Unless otherwise specified, analytically-pure reagents and Class-II water defined in compliant to GB/T 6682 are adopted for the purpose of the analysis.

4.1 Acetonitrile, Grade HPLC.

4.2 Methanol, Grade HPLC.

GB/T 5
QC/T
Foreword i
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Specimen preparation
7 Test procedure
8 Calculation of results
9 Lower determination limit and precision of the method
10 Test report
Annex A (Informative) HPLC-DAD detection wavelength and retention time of 11 carcinogenic dyestuffs
Annex B (Informative) HPLC-DAD chromatograms of standard samples of carcinogenic dyestuffs at different detection wavelengths
Annex C (Informative) HPLC-DAD spectrograms of standard samples of 11 carcinogenic dyestuffs
Annex D (Informative) Mass spectrometry reference parameters for 11 carcinogenic dyestuffs
Annex E (Informative) Multi-reactions monitoring (mrm) chromatograms of 11 carcinogenic dyestuffs