GB/T 31793-2015 in English
VALIDDetection and identification of Leptosphaeria maculans(Fuckel)Ces.et De Not.
- Issued on:2015-07-03
- Implemented on:2015-11-27
- File Format:PDF
- Delivery:Via email within 1~3 business days
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《GB/T 31793-2015油菜茎基溃疡病菌检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
本标准规定了油菜茎基溃疡病菌的检疫鉴定方法。
本标准适用于油菜和油菜茎基溃疡病菌其他十字花科寄主种子和植株中油菜茎基溃疡病菌的检疫和鉴定。
Introduction
1. Scope
This standard applies to the quarantine and identification of the pathogen of rapeseed stem canker in rapeseed and its host seeds and plants.
2. Instruments and Equipment
| Category | Instrument/Equipment Name | Purpose |
|---|---|---|
| Electronic Balance | Sensitivity 0.01g | Precisely weigh samples |
| Biological Microscope | - | Observe the morphology of hyphae and spores |
| PCR Instrument | - | Amplify target DNA fragments |
3. Reagents and Culture Medium
The main reagents include 1.0% sodium hypochlorite, agar powder, potatoes, glucose, etc. The culture medium is divided into potato dextrose agar (PDA) and acid potato dextrose agar (APDA).
4. Quarantine identification method
Seed detection process
- Pick abnormal seeds for isolation and culture.
- After sieving, take samples for initial PCR test.
- Positive samples are further isolated and cultured to confirm the results.
5. Identification characteristics
Identification is combined with morphological and molecular biological methods (such as PCR detection) to ensure accurate identification of pathogens.
6. Implementation suggestions
- The laboratory needs to be equipped with instruments and reagents specified in the standards.
- Operators should receive professional training.
- Calibrate equipment regularly and update test methods.
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