GB/T 31807-2015 in English
VALIDDetection and identification of Phymatotrichopsis omnivora(Duggar) Hennebert
- Issued on:2015-07-03
- Implemented on:2015-11-27
- File Format:PDF
- Delivery:Via email within 5 business days
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《GB/T 31807-2015棉花根腐病菌检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
本标准规定了棉花根腐病菌的检疫鉴定方法。
本标准适用于棉花根腐病寄主植物的植株、种子及介质的检疫鉴定。
Introduction
Standard Background and Technical Evolution
This standard was first published in 2015 to establish a systematic identification method for the cotton root rot pathogen (Phymatotrichopsis omnivora), an international quarantine pathogen. The pathogen originates from the southwestern United States and can infect more than 2,300 dicotyledonous plants, posing a major threat to my country's cotton industry.
Comparison of core identification technologies
| Identification dimensions | Traditional morphology | Molecular detection | Comprehensive judgment |
|---|---|---|---|
| Detection target | Mycelial cord/sclerotium morphology | 627bp specific fragment | Morphological + molecular double verification |
| Sensitivity | Visible signs required | Detectable trace DNA | Highest reliability |
| Time required | 5-7 days | 8 hours | 5-7 days |
Key identification characteristics
Typical symptoms
The surface of the diseased roots is covered with brown hyphae, the cortex is rotten and easily peeled off, reddish-brown lesions appear in the mid-column, and black sclerotia with a diameter of 1-5mm can be seen.
Morphological indicators
- Colony: initially white on PDA medium, then turns to dirty yellow
- Conidia: spherical/oval, 4.8-8μm in diameter
- Mycelial cords: brown, with right-angled branches
Implementation suggestions
- The wet sieving method should be used preferentially at the quarantine site to separate sclerotia from the medium
- Suspected samples should be isolated and cultured and tested by PCR (primers PoITS-A/B) at the same time
- Positive strains should be stored at -80℃ and the electrophoresis pattern should be recorded
Technical extension
This standard innovatively combines CTAB DNA extraction with specific primers, which increases the efficiency by 300% compared with the traditional culture method. The verification experiment in 2019 showed that the detection limit of seed samples reached 0.1% of the bacterial rate.
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