GB/T 33035-2016 in English
VALIDDetection and identification of Narcissus yellow stripe virus
- Issued on:2016-10-13
- Implemented on:2017-05-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 33035-2016水仙黄条病毒检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
1. Background and significance of standard formulation
Narcissus yellow stripe virus (NY SV) is a pathogen that seriously affects the quarantine safety of narcissus and its products. The formulation of this standard aims to provide a scientific basis for entry-exit inspection and quarantine work and ensure the quality and safety of plant products in international trade. The main drafting units of the standard include the Fujian Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the China Institute of Inspection and Quarantine and other authoritative institutions.
2. Comparison of technical frameworks of quarantine identification methods
| Detection method | Features | Applicable scenarios | Sensitivity |
|---|---|---|---|
| PT AE LIS A | Rapid initial screening, easy operation | Preliminary identification and large-scale screening | High |
| R TP C R | High sensitivity and good specificity | Confirmatory detection of positive samples | Extremely high |
| Real-time fluorescence R TP C | Strong quantitative ability, intuitive results | Accurate detection and quantitative analysis | Highest |
3. Practical application case analysis
Case: Quarantine and identification process of a batch of narcissus products
- Sampling method: Random sampling in accordance with SN / T 2122.
- Sample preparation: Symptomatic and asymptomatic samples are processed separately to ensure the representativeness and accuracy of the samples.
- PT AE LISA initial screening: Positive results need to be further verified by RTP CR or real-time fluorescence RTP CR.
- Final judgment: The sample test result is negative and does not carry narcissus yellow stripe virus.
4. Implementation Suggestions
- Laboratory conditions: Ensure that key equipment such as PCR instruments and fluorescent PCR instruments are equipped.
- Staff training: Regularly organize quarantine and testing technical training to improve operational standardization.
- Data records: Strictly preserve samples and test results in accordance with standard requirements to ensure traceability.
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