GB/T 33108-2016 in English
VALIDDetermination of sea cucumber glycosides in sea cucumber and sea cucumber products一 High performance liquid chromatography
- Issued on:2016-10-13
- Implemented on:2017-05-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$156.00
《GB/T 33108-2016海参及其制品中海参皂苷的测定 高效液相色谱法》由TC156（全国水产标准化技术委员会）归口，TC156SC3（全国水产标准化技术委员会水产品加工分会）执行，主管部门为农业农村部。
Introduction
National Standard of the People's Republic of China GB/T 33108—2016
This standard specifies the high performance liquid chromatography method for the determination of sea cucumber saponin content in sea cucumbers and their products. It is applicable to a variety of samples such as fresh sea cucumbers, dried sea cucumbers, ready-to-eat sea cucumbers and salted sea cucumbers.
| Sea cucumber species | Conversion factor F | Scope of application |
|---|---|---|
| Sea cucumber (Apostichopus japonicus) | 8.5 | Fresh and dried samples |
| Holothuria atra | 7.2 | Liquid products such as sea cucumber oral solution |
| Black sea cucumber (Holothuria atra) | 7.3 | Ready-to-eat products |
Principle and steps of the determination method
High performance liquid chromatography (HPLC) is the core technology of this standard. The quantitative analysis of sea cucumber saponins is achieved through the following steps:
- Sample preparation:Pre-treatment is performed according to the sample type, including homogenization, crushing and other steps.
- Extraction and purification:Sea cucumber saponins are extracted by ethanol-n-butanol extraction method, and purified by multiple extractions with chloroform.
- Hydrolysis and derivatization reaction:Trifluoroacetic acid is used to hydrolyze the saponin sugar chain, followed by a specific derivatization reaction with PMP reagent.
- Chromatographic analysis:The target product is separated by a C18 chromatographic column, and its ultraviolet signal is detected at 250nm.
Background and significance of the standard
This standard was proposed by the Ministry of Agriculture of the People's Republic of China and aims to unify the determination methods of active ingredients in sea cucumber products. By establishing a unified quality control system, it will help improve product quality and protect consumer rights.
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