GB/T 33249-2016 in English
VALIDNanotechnology--Quantification of gold nanorods in living cells--Extinction spectroscopy
- Issued on:2016-12-13
- Implemented on:2017-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 33249-2016纳米技术 活细胞内金纳米棒含量测定 消光光谱法》由TC279（全国纳米技术标准化技术委员会）归口，主管部门为中国科学院。
Introduction
1. Background and significance of the standard
Gold nanorods (AuNRs) have important application value in the biomedical field due to their unique physical and chemical properties. With the rapid development of nanotechnology, quantitative measurement of the content of gold nanorods in living cells has become a key indicator for evaluating their biocompatibility and therapeutic effects. This standard is based on the extinction characteristics of surface plasmon resonance (SPR) and provides a fast and accurate method suitable for studying the aggregation state of gold nanorods in cells and their concentration analysis.
2. Comparison table of standard frameworks
| Standard dimensions | GB/T33249-2016 | Reference standards |
|---|---|---|
| Scope of application | Determination of gold nanorod content in living cells | GB/T24369.1-2009 (UV/visible/near-infrared absorption spectroscopy method) |
| Core principles | Extinction spectroscopy, based on transverse and longitudinal surface plasmon resonance characteristics | Experimental verification method based on literature research |
| Measurement accuracy | Peak area is positively correlated with concentration, and the linear range is wide | Traditional ICP-MS method is used as a reference |
3. Analysis of standard implementation steps
3.1 Preparation process
ELISA is a key device, and its wavelength scanning range needs to cover 400nm to 999nm. The preparation of gold nanorods adopts the seed modulation growth method, and the aspect ratio of gold nanorods is precisely controlled by controlling the molar ratio of hexadecyltrimethylammonium bromide, tetrachloroauric acid and ascorbic acid.
3.2 Measurement and calculation
The background spectrum of the cell lysate needs to be deducted during measurement, and the gold nanorod content is calculated by peak area integration. Referring to the standard curve (Figure 3), the peak area can be converted into the actual concentration value. The number of parallel samples should be no less than 3 to ensure data reliability.
4. Implementation Suggestions
- Equipment Calibration: Regularly calibrate the microplate reader to ensure measurement accuracy.
- Sample Processing: Strictly control cell count and lysate concentration to avoid interference factors.
- Data Verification: It is recommended to use ICP-MS method for result verification (Appendix D).
Loading PDF document...
Error loading PDF. Please make sure the file is valid and try again.
