GB/T 33681.1-2017 in English

VALID

Methods to prepare samples for high-throughput gene sequencing—Part 1：Preparing samples of animal tissues

Issued on:2017-05-12
Implemented on:2017-12-01
File Format:PDF
Delivery:Via email within 2~4 business days

Price(USD)： $359.00
$349.00

Standard No:	GB/T 33681.1-2017
Document status:	VALID
Title in English:	Methods to prepare samples for high-throughput gene sequencing—Part 1：Preparing samples of animal tissues
Title in Chinese:	高通量基因测序样本预处理方法第1部分：动物组织样本预处理
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 2~4 business days
Issued on:	2017-05-12
Implemented on:	2017-12-01
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	A40-Basic Subject in general
Professional Classification:	GB-National Standard
Related Keywords:	animal tissue samples animal tissue sample pretreatment methods high-throughput gene animal tissues introduction interpretation fragmentation methods technical
Related Topics:	sample tissue processor preprocessing sequencing sample standard Automatic sample preparation for pathological samples atomic preprocessing high-throughput tissue streaming tissue processing Sample Handling Peak Southern High-throughput Organization animal tissue test sample sample Sampling is related to preprocessing Sample preconditioner Gene Sequencing Animal Sample Handling Biological sample pretreatment tissue sample Inorganic sample treatment method Sample processing method High throughput sampler Test sample handling method Water sample pretreatment automatic tissue processing Methods of sample pretreatment sampler Multi-sample genome analysis Sample Processing Processing Method micro sample sequencing What to do with modulus of elasticity Sequencing samples Sequencing samples high-throughput tissue Sample preparation for texture measurement How to handle samples Trace moisture pretreatment instrument pretreatment Tissue Disposal Box Lao preprocessor Mercury pretreatment Water sample preconditioner Two treatments of the same sample Sample No Handling Method Sample pretreatment Is targeted sequencing the same as gene sequencing Fundamentals of spectral processing Pretreatment series Handle small samples Sample Processor Biological sample pretreatment method Solid pretreatment method Sample processing method Water sample preconditioner tissue sample Sample Pretreatment Principles Solid pretreatment method Sample handling box Sample Pretreatment Principles processed as a sample 6 pretreatment methods for samples streaming tissue processing Biomass pretreatment Biomass pretreatment Four kinds of biological pretreatment Animal Gene Sequencing Processing method Preprocessing Principle of sample processing Pre-column test sample Are u6 genes the same? Sample Processing Method Method animal gene Water sample pretreatment Sequencing sample size some tissue genes pretreatment agent animal sequencing Monitor sample processing Tissue sample volume sample place How to split the peak Inorganic sample handling Sample Measurement Method how about pretreatment agent Test set preprocessing Sample processing High-throughput sequencing of tissues High-throughput sequencing samples Sample Handling Version Lao preprocessor role Organic pretreatment Inorganic pretreatment genetic animal method animal sequencing Test sample pretreatment Quickly process samples group sample method Animal Tissue Processing What to do with the baseline sequencing Tissue Sequencing Methods tissue gene basic analysis sample Micro-sampling of samples animal gene method Measurement preprocessing GB/T 33681.1 GB/T 33681.1-2017 Sample pretreatment Necrosulfonamide pretreatment 1h Gas Chromatography Sample Pretreatment DR preprocessing Basic procedures for gene cloning How to deal with high mass spectrum baseline Basic methods and treatment processes of sewage treatment Unexpected test of sample sequencing Gene sequencing analysis methods Basic methods of heat treatment What are the methods for preprocessing experimental samples? 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Different processing methods and times for the same sample Water sample pretreatment and methods New food analysis preprocessing method Electrophoresis sample processing methods Skin tissue sample pre-processing methods What are the sample pretreatment methods? Near infrared pretreatment method HPLC sample processing methods Methods of saccharification pretreatment Factory Sample Processing Method Clean bench specimen processing method

《GB/T 33681.1-2017高通量基因测序样本预处理方法第1部分：动物组织样本预处理》由TC387（全国生化检测标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

Interpretation of animal tissue sample pretreatment methods for high-throughput gene sequencing

1. Standard Overview

GB/T 33681.1—2017 mainly regulates the pretreatment process of animal tissue samples in high-throughput gene sequencing technology, covering the entire process from nucleic acid extraction to single molecule amplification.

1.1 Background of Standard Formulation

With the rapid development of high-throughput gene sequencing technology, the quality requirements for sample pretreatment are increasing. This standard is managed by the China Association for Standardization to ensure the standardization and repeatability of the experimental process.

2. Comparison of standard frameworks

d>Ensure the accuracy of experimental results

Standard dimensions	GB/T 33681.1—2017	Related international standards	Advantage analysis
Pretreatment scope	Multiple types of samples such as blood and fresh tissues	Mainly for specific sample types	Wider applicability and unified operating procedures
Technical requirements	Clear laboratory conditions and instrument parameters	Greater flexibility
Quality assessment	Detailed provisions for nucleic acid purity and concentration standards	Evaluation indicators are not comprehensive	Provide more scientific quality control methods

3. Nucleic acid extraction and fragmentation

Nucleic acid extraction:Phenol-chloroform method and anionic detergent method are commonly used. Attention should be paid to removing impurities such as proteins to ensure the purity of DNA/RNA.

3.1 Comparison of fragmentation methods

Technical means	Applicable scenarios	Operation steps	Advantages and disadvantages
Enzyme digestion	Genomic DNA research	Select restriction endonucleases to cut specific sites	High specificity, but enzyme concentration needs to be optimized
Ultrasonic method	Small RNA library construction	Adjust power and time to control fragment length	Flexible operation, but nucleic acid degradation needs to be prevented

4. Analysis of single molecule amplification technology

Emulsion PCR (EPCR): Single molecule amplification is performed in an oil-in-water emulsion, which is suitable for generating sequencing libraries with high uniformity.

4.1 Suggestions for optimizing experimental steps

Case analysis: Problems and solutions in EPCR operation

Problem: Too many bubbles appear during the emulsion preparation process.
Solution: Ensure that the reagents are fully mixed before using a vortex oscillator to reduce violent shaking.

5. Implementation suggestions

Laboratory construction: According to the sample type and processing volume, rationally plan the functional zoning to ensure compliance with biosafety requirements.

Staff training: Focus on training nucleic acid extraction and single molecule amplification technology to enhance biosafety awareness.

Quality Control: Regularly calibrate instruments and equipment, conduct quality assessments in strict accordance with standards, and ensure the traceability of each batch of samples.

More samples: NB/T 47013.2-2015 JJG 21-2008 JGJ 94-2008

Sample only — not a preview of GB/T 33681.1-2017

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