GB/T 34728-2017 in English

VALID

PCR for Mycoplasma agalactiae

Issued on:2017-11-01
Implemented on:2018-05-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 34728-2017
Document status:	VALID
Title in English:	PCR for Mycoplasma agalactiae
Title in Chinese:	无乳支原体PCR检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-11-01
Implemented on:	2018-05-01
ICS Classification:	11.220-Veterinary medicine
Chinese Classification:	B41-Animal quarantine, veterinary and epidemic prevention
Professional Classification:	GB-National Standard
Related Keywords:	mycoplasma agalactiae standard formulation mycoplasma agalactiae mycoplasma agalactiae nucleic acid pcr detection method standard agalactiae introduction interpretation
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《GB/T 34728-2017无乳支原体PCR检测方法》由TC181（全国动物卫生标准化技术委员会）归口，主管部门为农业农村部。

Introduction

Interpretation of the PCR Detection Method Standard for Mycoplasma agalactiae

1. Background and Significance of Standard Formulation

Mycoplasma agalactiae (Mycoplasma agalactiae) is the main pathogen that causes mastitis, arthritis and conjunctivitis in sheep, and seriously threatens the healthy development of animal husbandry. In order to standardize the detection method and ensure the accuracy and consistency of the test results, the Ministry of Agriculture of the People's Republic of China organized the drafting of the GB/T 34728-2017 standard. This standard combines the current advanced PCR technology at home and abroad and provides a scientific basis for the rapid diagnosis of Mycoplasma agalactiae.

2. Overview of the Detection Method

This standard specifies the complete operating procedures from sample collection to PCR amplification and result determination. It is suitable for detecting Mycoplasma agalactiae nucleic acid in clinical samples and isolated cultures of sheep (goats and sheep).

Standard dimensions	Domestic standards	International standards	Characteristics of this standard
Detection sensitivity	≥95%	≥90%	Optimized to 98%, improving detection accuracy
Specificity	≥98%	≥95%	Introducing high-specificity primers to avoid cross-reactions
Detection time	4-6 hours	5-8 hours	Process optimization, saving 1 hour of operation time

3. Detailed interpretation of the detection method

Sample collection and processing:According to different clinical symptoms, milk samples, joint fluid, eye swabs or tissue samples are collected respectively. All samples must be sterile and sent to the laboratory for testing as soon as possible after collection.

Actual application case

A large-scale sheep farm had a mastitis epidemic, and 20 milk samples were tested by PCR using this standard method. The results showed that 18 samples were positive, with an accuracy rate of 90%, which was consistent with the laboratory culture results, proving the effectiveness of this method.

4. Implementation suggestions

Staff training:Laboratory technicians need to receive special training on PCR operation and biosafety.
Equipment maintenance:Regularly calibrate key equipment such as PCR instruments and electrophoresis instruments to ensure detection accuracy.
Reagent management: Prepare reagents strictly in accordance with standard requirements and perform batch comparison to ensure consistency of results.

More samples: GB 18306-2015 JJG 21-2008 NB/T 47013.2-2015

Sample only — not a preview of GB/T 34728-2017

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