GB/T 34745-2017 in English

SUPERSEDED

Porcine circovirus type 2-Method of detecting virus by SYBR Green Ⅰreal-time PCR

Issued on:2017-11-01
Implemented on:2018-05-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 34745-2017
Document status:	SUPERSEDED
Superseded by:	GB/T 21674-2025 Diagnostic techniques for porcine circovirus disease
Superseded on:	2026-03-01
Title in English:	Porcine circovirus type 2-Method of detecting virus by SYBR Green Ⅰreal-time PCR
Title in Chinese:	猪圆环病毒2型病毒SYBR GreenⅠ实时荧光定量PCR检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-11-01
Implemented on:	2018-05-01
ICS Classification:	11.220-Veterinary medicine
Professional Classification:	GB-National Standard
Related Keywords:	porcine circovirus type ⅰreal-time pcr introduction interpretation pcr laboratory environment control porcine circovirus pcr laboratory
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《GB/T 34745-2017猪圆环病毒2型病毒SYBR GreenⅠ实时荧光定量PCR检测方法》由TC181（全国动物卫生标准化技术委员会）归口，主管部门为农业农村部。

Introduction

Interpretation of GB/T 34745—2017 National Standard of the People's Republic of China

Standard Dimensions	Technical Requirements	Scope of Application	Comparative Analysis
Reagent Preparation	All reagents must be packaged in containers without RNAse contamination and sterilized at high temperature.	Applicable to PCR laboratory environment control.	Compared with traditional PCR methods, it emphasizes higher cleanliness requirements.
Instruments and Equipment	Must be equipped with key equipment such as fluorescent quantitative PCR amplifiers and high-speed refrigerated centrifuges.	Applicable to high-precision molecular biology detection.	The requirements for equipment performance have been significantly improved to ensure data accuracy.
Sample processing	Includes standardized processes such as tissue grinding and DNA extraction.	Applicable to clinical sample testing of suspected infected pigs.	The extraction steps have been optimized to improve detection sensitivity.

Background of standard formulation and analysis of technological evolution

Background:Porcine circovirus type 2 (PCV2) is an important pathogen that causes post-weaning multisystemic wasting syndrome (PMWS) in piglets, causing huge economic losses to the pig industry. Real-time fluorescence quantitative PCR technology has become the preferred method for detecting PCV2 due to its high efficiency and sensitivity.

Technological evolution: From the initial conventional PCR to real-time fluorescence quantitative PCR, the detection efficiency has been improved by several orders of magnitude. The SYBR Green I dye method overcomes the problems of high cost and poor specificity of the traditional probe method, and significantly lowers the detection threshold.

Implementation suggestions

Laboratory conditions: Ensure that the PCR laboratory meets the biosafety level 2 and above standards and is equipped with corresponding protective equipment.
Staff training: Operators must undergo professional training and master the skills of reagent preparation, instrument operation and data analysis.
Quality control management: Strictly control the frequency of use of negative controls and positive controls to ensure the validity of experimental results.
Data records: It is recommended to establish an electronic data management system to facilitate tracking and data analysis.