GB/T 35335-2017 in English
VALIDGuidelines for surveillance of Cucumber green mottle mosaic virus
- Issued on:2017-12-29
- Implemented on:2019-01-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$156.00
《GB/T 35335-2017黄瓜绿斑驳花叶病毒病监测规范》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
Interpretation of the Monitoring Standards for Cucumber Green Mottle Mosaic Virus
1. Background and Significance of Standard Formulation
Cucumber green mottle mosaic virus (CGMMV) is a disease that seriously affects cucurbit crops. Its transmission pathway is complex and its damage is serious. This standard is based on the detection method of NY/T 2288—2012 and is formulated in combination with actual monitoring experience. It aims to standardize the epidemic monitoring process and ensure agricultural safety production.| Dimensions | Nursery monitoring | Monitoring of non-occurring areas | Monitoring of affected areas |
|---|---|---|---|
| Monitoring focus | High-risk seedling breeding bases | Areas with high risk of epidemic importation | Representative plots and marginal areas |
| Monitoring frequency | Survey each nursery and each plant | 2-3 inspections during the growing season | Fixed-point inspections every 7 days |
| Sampling ratio | 20%-30% | 20%-30% | 10 survey points, 10 plants randomly surveyed at each point |
2. Implementation suggestions and case analysis
Practical application:After suspected symptoms are found in a certain place, preliminary identification is carried out through rapid immunoassay test strips, and the epidemic is confirmed by laboratory testing. The fixed-point survey method is used to record the diseased plant rate and diseased fruit rate to form a complete monitoring report.
| Crop Name | Growth Period | Survey Area (mu) | Number of Diseased Plants |
|---|---|---|---|
| Watermelon | Fruits Period | 50 mu | 20 Plants |
| Cucumber | Vine Extending Period | 30 mu | 15 Plants |
3. Recommendations for Specimen Preservation and Processing
The materials and utensils used in the monitoring process should be disinfected. Seedling samples need to be stored in a 4°C refrigerator, and leaf and fruit samples should be freeze-dried and stored below -20°C for future reference. After the storage period expires, inactivation treatment is required to ensure biosafety.
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