GB/T 35537-2017 in English

VALID

Requirements of the high-throughput gene sequencing result evaluation

Issued on:2017-12-29
Implemented on:2018-07-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 35537-2017
Document status:	VALID
Title in English:	Requirements of the high-throughput gene sequencing result evaluation
Title in Chinese:	高通量基因测序结果评价要求
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2017-12-29
Implemented on:	2018-07-01
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	A40-Basic Subject in general
Professional Classification:	GB-National Standard
Related Topics:	Qualcomm sequencing Sequencing results xrf measurement results xrf measurement results GB/T 35537-2017 high-throughput evaluation Gene number result half height How to combine the sequencing results with the original results Gene Sequencing GB/T 35537 Content result en result evaluation Methods of evaluating results gene number gene Requirements for evaluation of high-throughput gene sequencing results GB/t+35537 Sequencing result peak High-throughput gene sequencing6 high-throughput gene Evaluation of the results Evaluation of test results Gene sequencing results Result evaluation and inspection Sequencer result evaluation Evaluation of the results Evaluation of test results Result evaluation and analysis gene sequencer GM results High throughput sequencer s5 The second generation of high-throughput sequencing gene sequencer Compare evaluation results result evaluation Measurement Results System Gene sequencing results s5 high-throughput sequencer Sequencing results forward and reverse a gene sequence Is targeted sequencing the same as gene sequencing Evaluation Results and Analysis High-throughput gene sequencing Evaluation result analysis Secondary results of gene sequencing In-Gene Sequencing genetic result gene sequencing forty Evaluation Results and Analysis Animal Gene Sequencing gene sequencer high frequency gene Evaluation of measurement results gene sequencing to How Genetic Sequencing High-throughput sequencing s5 High-throughput sequencing results Upstream gene Downstream gene base a Evaluate test results High-throughput sequencing of tissues Method of evaluating results Measurement Effect Evaluation Evaluation of sequencing results two-factor evaluation One-stop high-throughput sequencing Upstream Genes and Downstream Genes Methods of evaluating the measurement results gene sequencer Double evaluation evaluation factor gene sequencing set Test Evaluation Results High-throughput sequencing triploids nearby genes high result Results and Evaluation Gene sequencing at home and abroad Three types of high-throughput sequencing sequencing Evaluate test results Comparison of evaluation results Basis for evaluation of test results Multi-gene high-throughput high-throughput sequencing 30 years of gene sequencing The past and present of high-throughput sequencing cluster gene sequencing gene sequencing, gene, sequencing genetic testing, gene, gene sequencing, sequencing first gene to be sequenced Method for evaluating measurement results Peptide sequencing results Methods of outcome evaluation The Past and Present of Gene Sequencing Sequencing result nesting Gene sequencing past and present 4th Generation Gene Sequencing Two high-throughput sequencing How to evaluate the test results factor result most widely sequenced gene gene linkage High-throughput target gene sequencing Calorimeter results Evaluate Heiguo GBT35537 GB/T 35537-2017 Sequencing test fails, test passes Analysis of illumina sequencing results Analysis of monoclonal antibody sequencing results Basic procedures for gene cloning gene pathways HPV gene sequencing method LUC gene The qPCR results are opposite to the WB results High-throughput sequencing experimental steps The test result is not passed. Sequencing requirements are unexpected Optimal results for gene assembly LPA gene Gene sequencing requirements for laboratories Gene sequencing analysis methods High-throughput sequencing genotyping system General procedures for gene sequencing Evaluation of standard instrument traceability results Test conclusion and evaluation Evaluation of method comparison results

《GB/T 35537-2017高通量基因测序结果评价要求》由TC387（全国生化检测标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

Interpretation of the evaluation standard for high-throughput gene sequencing results

1. Background of standard formulation and analysis of technological evolution

With the rapid development of gene sequencing technology, high-throughput gene sequencing has become an important tool for biomedical research and clinical diagnosis. This standard was created to ensure the quality and consistency of sequencing results.

1.1 Background of Standardization

Standardization requirements based on existing technologies
Unify the industry evaluation indicator system
Improve the reliability and comparability of sequencing results

2. Comparison and interpretation of standard frameworks

Evaluation dimensions	Ligase sequencing requirements	Polymerase sequencing requirements
Sequencing throughput	≥500M reads	≥3M reads
Base sequencing accuracy	≥99.0%	≥99.0%
Average sequencing length	≥50bp	≥35bp

3. Implementation suggestions and actual cases

3.1 Implementation suggestions

Choose a suitable sequencing method:Based on research needs and budget, choose the ligase method or polymerase method reasonably.
Ensure that reagents match instruments:Use validated reagent kits and consumables to avoid compatibility issues.
Regularly calibrate equipment:Maintain and calibrate the sequencer according to the manufacturer's recommendations to ensure data accuracy.

More samples: DL/T 368-2010 GB 50021-2001(2009) QC/T 942-2021

Sample only — not a preview of GB/T 35537-2017

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