GB/T 35906-2018 in English

VALID

Indirect ELISA to detect antibody against classical swine fever virus

Issued on:2018-02-06
Implemented on:2018-09-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 35906-2018
Document status:	VALID
Title in English:	Indirect ELISA to detect antibody against classical swine fever virus
Title in Chinese:	猪瘟抗体间接ELISA检测方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2018-02-06
Implemented on:	2018-09-01
ICS Classification:	11.220-Veterinary medicine
Chinese Classification:	B41-Animal quarantine, veterinary and epidemic prevention
Professional Classification:	GB-National Standard
Related Keywords:	classical swine fever virus introduction interpretation application classic swine fever antibody detection method specially classical swine fever antibodies swine fever vaccines indirect elisa
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《GB/T 35906-2018猪瘟抗体间接ELISA检测方法》由TC181（全国动物卫生标准化技术委员会）归口，主管部门为农业农村部。

Introduction

Interpretation of the National Standard of the People's Republic of China GB/T 35906—2018

Background and Significance of the Standard

This standard was proposed by the Ministry of Agriculture of the People's Republic of China, and is under the unified management of the National Technical Committee for Animal Health Standardization. The main drafting unit is the China Veterinary Drug Administration. The formulation of this standard aims to standardize the detection method of classical swine fever antibodies and ensure the scientificity and accuracy of the evaluation of vaccine immunization effects.

Standard Dimensions	GB/T 35906—2018	Comparative Analysis
Scope of Application	Classic swine fever antibody detection method	Specially used for antibody detection after vaccine immunization, not for individual differential diagnosis.
Technical requirements	Indirect ELISA method	Standardized operation steps to ensure repeatability and accuracy of results.
Reagents and equipment	Specified technical parameters of key reagents such as E2 protein and enzyme conjugate	In line with international standards to ensure comparability of test results.

Actual case analysis of standard implementation

In actual application, this standard has been widely used in the evaluation of immunization effects of swine fever vaccines in large and medium-sized farms. For example, a large pig farming enterprise significantly improved the accuracy of determining the antibody positive rate by adopting the indirect ELISA test method specified in this standard, thereby optimizing the immunization program.

Implementation Suggestions

Laboratory conditions:Ensure that the necessary equipment such as microplate reader and 37℃ incubator are available.
Staff training:Operators must undergo professional training and be familiar with reagent preparation and testing procedures.
Quality control:Regularly calibrate the instrument and strictly follow the standard operating procedures.

More samples: JJG 21-2008 YD/T 3934-2021 GB 18306-2015

Sample only — not a preview of GB/T 35906-2018

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