GB/T 36815-2018 in English

VALID

Detection and identification of Diaporthe vaccinii Shear

Issued on:2018-09-17
Implemented on:2019-04-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $250.00
$243.00

Standard No:	GB/T 36815-2018
Document status:	VALID
Title in English:	Detection and identification of Diaporthe vaccinii Shear
Title in Chinese:	蓝莓果腐病菌检疫鉴定方法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2018-09-17
Implemented on:	2019-04-01
ICS Classification:	65.020.01-Farming and forestry in general
Chinese Classification:	B16-Plant quarantine, pest control
Professional Classification:	GB-National Standard
Related Keywords:	diaporthe vaccinii diaporthe vaccinii shear introduction interpretation quarantine identification methods quarantine identification molecular biological detection
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《GB/T 36815-2018蓝莓果腐病菌检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

Interpretation of quarantine identification methods for blueberry fruit rot pathogens

Pathogen	Cultivation characteristics on PDA	Characteristics of conidia (measurement results on the host)	Characteristics of conidia (measurement results on the host)
Diaporthe vaccinii	The hyphae are loose, white to light gray-brown, grow radially, are woolly, and have annular bands. After 3 weeks, some strains will produce gray-white fungus masses	Conidiophores are spherical, with a wide base, buried, scattered or connate, simple in cavity, with a single conidial opening, 200μm high and 500μm wide, conidiophores are short, septate, branched, and the conidial cells are simple, tapering at the top, sometimes spindle-shaped	α spores (6.0μm～10.5μm)× (2.2μm～3.2μm), unicellular, transparent, spindle-shaped, pointed at both ends, with 2 oil balls
D. eres	Mycelium is white, cottony or wooly	Conidiophores are spherical, scattered, buried, simple in cavity, with a single conidial opening, 290μm～300μm	α spores (6.5μm～8.0μm)× （2.3μm～3.2μm）, unicellular, transparent, oblong, sometimes blunt at one end, slightly curved at one end, with 1 or 2 oil balls
D. viticola	The edge of the colony is complete or slightly wavy, the hyphae are cotton-like, white, and in the late growth stage, there are orange conidial droplets on the surface of the colony	The conidiophores are born on the surface of the stroma, which is not obvious, initially disc-shaped, and becomes spherical after maturity, with a single cavity, a short neck, and an irregular opening at the top	α spores (13.0μm～17.0μm)× (2.2μm～3.0μm), unicellular, transparent, spindle-shaped, pointed at both ends, with multiple oil balls

Implementation suggestions and actual case analysis

In actual operation, it is recommended to follow the following steps to carry out quarantine identification of blueberry fruit rot pathogens:

Sample collection and processing:Select branches, leaves or fruits with suspicious symptoms as test samples, and ensure that the samples are properly preserved.
Direct microscopic examination:After the conidiophores are found, microscopic observation is immediately carried out, and the morphological characteristics of the conidia are recorded.
Moisture-retentive culture:Moisture-retentive culture is carried out on samples with suspicious symptoms to induce the pathogen to produce conidiophores.
Isolation and culture:Inoculate the sample onto APDA selective culture medium, observe the colony morphology and purify it.
Molecular biological detection:After extracting DNA, use ITS1/ITS4 primers to amplify the target fragment and compare it with the GenBank database to confirm the identity of the pathogen.

More samples: JJG 21-2008 JGJ 94-2008 JC/T 2453-2018

Sample only — not a preview of GB/T 36815-2018

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