GB/T 36816-2018 in English
VALIDDetection and identification of Potato virus Y
- Issued on:2018-09-17
- Implemented on:2019-04-01
- File Format:PDF
- Delivery:Via email within 5 business days
$301.00
《GB/T 36816-2018马铃薯Y病毒检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
GB/T 36816—2018 Standard Overview
This standard specifies in detail the quarantine identification methods for Potatovirus Y (PVY), and is applicable to potato plants, propagation materials and other host plants that may carry the virus.
Background of Standard Formulation
Potatovirus Y is a quarantine disease of global concern, and its transmission pathways are diverse, including asexual propagation materials and mechanical inoculation. This standard was proposed by the National Technical Committee for Plant Quarantine Standardization and aims to provide unified technical specifications for domestic and international trade.
Principle and technical framework of the method
| Detection method | Principle | Scope of application |
|---|---|---|
| DAS-ELISA | Immunological method based on antigen-antibody reaction | Suitable for rapid qualitative detection |
| RT-PCR | Reverse transcription polymerase chain reaction, based on viral genome characteristics | Suitable for high sensitivity and specificity detection |
| Real-time RT-PCR | Real-time quantitative reverse transcription polymerase chain reaction, combined with fluorescent labeling | Used for quantitative analysis of viral load |
Comparison of standard frameworks
| Detection method | Advantages | Disadvantages |
|---|---|---|
| DAS-ELISA | Easy to operate and low cost | Low sensitivity, requires high-quality reagents |
| RT-PCR | High sensitivity and specificity | Complex operation and long cycle |
| Real-time quantitative reverse transcription polymerase chain reaction, combined with fluorescent labeling | Used for quantitative analysis of viral load | Real-time monitoring, quantitative analysis | Highly equipment-dependent |
Implementation recommendations
Reagent selection:Preferably use validated commercial kits to ensure detection sensitivity and specificity.
Sample processing:Strictly follow standard requirements for sample sampling and preparation to avoid contamination and operational errors.
Data recording:Record the experimental process and results in detail to ensure data traceability and integrity.
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