GB/T 36822-2018 in English
VALIDDetection and identification of Acidovorax citrulli
- Issued on:2018-09-17
- Implemented on:2019-04-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$243.00
《GB/T 36822-2018瓜类果斑病菌检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
GB/T 36822—2018 Quarantine and Identification Methods for Cucurbit Fruit Spot Pathogens
Standard Background and Significance
GB/T 36822—2018 Quarantine and Identification Methods for Cucurbit Fruit Spot Pathogens is an important agricultural standard, which is mainly used to regulate the quarantine and identification of Cucurbit Fruit Spot Pathogens (Acidovorax citrulli) in melon crops. This standard was proposed and coordinated by the National Technical Committee for Plant Quarantine and Standardization, aiming to ensure the quality and safety of melon products and prevent the spread of diseases through scientific and unified methods.
Comparison table of standard frameworks
| Standard dimensions | Scope of application | Technical requirements | Implementation points |
|---|---|---|---|
| Melon crops | Cucurbit crops such as watermelon and melon | Symptom observation and isolation culture combined with molecular biological detection | Combination of field observation and laboratory testing |
| Pathogen type | Melon fruit spot pathogen (Acidovorax citrulli) | PCR, real-time fluorescence PCR, padlock probe technology | Positive samples need to be isolated and cultured for confirmation |
| Detection methods | Plant and seed samples | Immunological and molecular biological tests | Results need to be determined by combining multiple detection methods |
Explanation of professional terms
Acidovorax citrulli: A bacterium that harms cucurbit crops, mainly spread through infected seeds. Its symptoms include water-soaked spots on leaves and fruit rot, which seriously affect the yield and quality of melons.
Instrument and reagent requirements
Instrument and equipment: Including PCR instrument, real-time fluorescence PCR instrument, electrophoresis instrument, etc. These devices play a key role in molecular biological detection, for example, PCR is used to amplify pathogen-specific DNA fragments.
Implementation suggestions
Field symptom observation and sample collection
For plants suspected of being infected with cucurbit fruit spot pathogen, detailed symptom observation and photo recording are required. Asymptomatic plants should also be randomly sampled to ensure comprehensiveness. Seed samples can be prepared by crushing and then soaking to extract the bacteria.
Loading PDF document...
Error loading PDF. Please make sure the file is valid and try again.
We also recommend
-
GB/T 35238-2017 in English
Technical specification for control of wheat stripe rust(Puccinia striiformis West.)
2017-12-29 -
GB/T 44619-2024 in English
Detection and identification of Pomacea Perry
2024-09-29 -
GB 7411-2009 in English
Quarantine protocol for cotton seeds in producing areas
2009-04-27 -
GB/T 15791-2011 in English
Rules of monitoring and forecasting for the rice sheath blight (Rhizoctonia solani Kukn)
2011-09-29 -
GB/T 28062-2025 in English
Code of practice for detection of Candidatus Liberibacter asiaticus by quantitative real-time PCR
2025-05-30 -
GB/T 28972-2012 in English
Export Certification System for Plant Quarantine
2012-12-31
