GB/T 36840-2018 in English
VALIDDetection and identification of Clavibacter michiganensis subsp. nebraskensis
- Issued on:2018-09-17
- Implemented on:2019-04-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 36840-2018玉米内州萎蔫病菌检疫鉴定方法》由TC271（全国植物检疫标准化技术委员会）归口，主管部门为国家标准化管理委员会。
Introduction
Quarantine identification method for corn wilt - in-depth analysis of national standards
| Test steps | Specific requirements | Technical specifications |
|---|---|---|
| Sample pretreatment | Pretreatment methods for seeds and plant materials | - Seed soaking: 200mL PBS buffer, 4°C overnight - Plant tissue sampling: 2mm×2mm at the junction of diseased and healthy tissues |
| PCR initial test | Rapid detection based on real-time fluorescence PCR | - Primer combination: PSA-7/PSA-R and 1184-F/1184-R - Amplification product length: 393bp and 390bp |
| Pathogen isolation | Colony purification based on culture medium | - Use sCNS and NA culture medium - Suspected colonies need to be purified 3 times |
Explanation of professional terms and practical application cases
PCR detection: Modern molecular biology technology, used to quickly identify the presence of pathogens.
Biolog test: Confirm the characteristics of the strain through microbial metabolic profile analysis.
Actual case: CMN-positive samples were detected in imported corn seeds and need to be destroyed in accordance with the standard.
Background of standard formulation and technological evolution
This standard is based on internationally accepted plant quarantine regulations and is formulated in combination with my country's actual situation. In recent years, with the development of molecular biology technology, PCR detection methods have gradually replaced traditional morphological identification and become the main detection method.
Implementation suggestions
- Strengthen laboratory personnel training to ensure that they master the latest detection technology.
- Establish an information sharing mechanism between regions and promptly report epidemic dynamics.
- Promote rapid detection equipment to improve port quarantine efficiency.
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