GB/T 37115-2018 in English
VALIDProtocol of diagnosis for infection with abalone herpersvirus
- Issued on:2018-12-28
- Implemented on:2019-07-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
$214.00
《GB/T 37115-2018鲍疱疹病毒病诊断规程》由TC156（全国水产标准化技术委员会）归口，TC156SC11（全国水产标准化技术委员会水产养殖病害防治分会）执行，主管部门为农业农村部。
Introduction
National Standard of the People's Republic of China GB/T 37115—2018
Interpretation of the Diagnostic Procedure for Abalone Herpes Virus Disease
| Detection Method | Overview of Steps | Advantages and Features |
|---|---|---|
| Histopathology Detection | Sampling, fixation, sectioning, staining and observation. | Visually display nerve tissue lesions, suitable for preliminary screening. |
| Real-time fluorescence PCR Detection | Sample processing, nucleic acid extraction, qPCR reaction and result determination. | High sensitivity and specificity, suitable for rapid diagnosis. |
| Traditional PCR test | Sample preparation, amplification, electrophoresis and sequencing analysis. | Verify the results of fluorescent PCR, suitable for subsequent confirmation. |
Background and significance of standard formulation
Abalone Herpesvirus (AbHV) is an infectious disease that seriously endangers the global abalone aquaculture industry, especially in Asia and Australia. This standard is based on a large number of epidemiological surveys and laboratory studies, and aims to provide a scientific basis for the prevention, monitoring and control of the disease.
Main technical content and framework
This standard covers the diagnostic methods of abalone herpes virus disease, including:
- Observation of clinical symptoms
- Histopathological examination
- Real-time fluorescence PCR (qPCR) detection
- Traditional polymerase chain reaction (PCR) detection
Implementation recommendations and practical applications
To ensure the effective implementation of the standard, it is recommended that:
- Strengthen technical training for grassroots laboratories.
- Establish a unified positive sample library for comparison and verification.
- Promote this diagnostic method in aquaculture areas.
Appendices and References
The standard contains several appendices, which explain in detail:
- Target gene reference sequence
- Reagent preparation method
- Clinical symptom description and pathological changes
Through the implementation of this standard, the diagnostic ability of abalone herpes virus disease can be effectively improved, economic losses can be reduced, and aquatic biodiversity can be protected.
Loading PDF document...
Error loading PDF. Please make sure the file is valid and try again.
We also recommend
-
GB/T 25878-2010 in English
Polymerase chain reaction(PCR) method for infectious hypodermal and haematopoietic necrosis virus(IHHNV)
2011-01-10 -
GB/T 36190-2018/XG1-2020 in English
Protocol of diagnostic methods for hemorrhagic disease of grass carp,includes Amendment 1
2020-12-14 -
GB/T 28630.1-2012 in English
Diagnostic protocols for white spot disease—Part 1:Dot blot hybridization method with DNA probe
2012-07-31 -
GB/T 20443-2006 in English
Method for the determination of diethylstilbestrol in chicken tissue by high performance liquid chromatography with electrochemical detection
2006-08-28 -
GB/T 42821-2023 in English
Diagnostic methods for bonamiosis of molluscs
2023-08-06 -
GB/T 41185-2021 in English
Specification of preparation and quality control of reference materials for DNA detection of aquatic animals pathogen―Plasmid
2021-12-31
