GB/T 37272-2018 in English
VALIDDetermination of 11--nor--9--carboxy--delta--9--tetrahydrocannabinol in urine--Liquid chromatography--tandem mass spectrometry method
- Issued on:2018-12-28
- Implemented on:2019-04-01
- File Format:PDF
- Delivery:Via email within 1~3 business days
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《GB/T 37272-2018尿液中△9-四氢大麻酸的测定 液相色谱-串联质谱法》由315（司法部）归口，主管部门为司法部。
Introduction
Standard Background and Significance
National Standard of the People's Republic of China GB/T 37272-2018 "Determination of Δ⁹-tetrahydrocannabinoid in urine by liquid chromatography-tandem mass spectrometry" is one of the important standards in the field of forensic identification in my country. The standard was proposed and managed by the Ministry of Justice, and the main drafting unit was the Institute of Forensic Science. The formulation of the standard aims to standardize the detection method of Δ⁹-tetrahydrocannabinoid (THC) in urine samples and ensure the accuracy and reliability of forensic identification results.
Comparative analysis of standard frameworks
| Standard dimensions | GB/T 37272—2018 | Internationally accepted standard |
|---|---|---|
| Detection method | Liquid chromatography-tandem mass spectrometry (LC-MS-MS) | Liquid chromatography-tandem mass spectrometry (LC-MS-MS), gas chromatography-mass spectrometry (GC-MS) |
| Scope of application | Urine samples | Various biological samples such as blood, urine, saliva, etc. |
| Sensitivity | The detection limit is 4 ng/mL, the lower limit of quantification is 10 ng/mL | Usually 1-5 ng/mL |
Instrument and technical points
Liquid chromatography-tandem mass spectrometry (LC-MS-MS)
This standard uses Liquid chromatography-tandem mass spectrometry, equipped with an electrospray ionization source (ESI). Its core advantages are:
- High sensitivity:Suitable for trace analysis.
- Multiple reaction monitoring (MRM): Improve detection specificity and sensitivity.
- Quantitative accuracy: Ensure the accuracy of the results through the internal standard method.
Implementation recommendations and precautions
To ensure the correct implementation of the standard, the following recommendations are made:
- Reagent selection: Use high-purity reagents and first-grade water to avoid interference.
- Instrument calibration: Regularly calibrate the mass spectrometer to ensure stable sensitivity.
- Sample processing: Strictly follow the extraction steps in the standard to avoid contamination.
- Data analysis: It is recommended to use professional software for peak area ratio calculation and linear regression analysis.
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