GB/T 37512-2019 in English

VALID

Inspection of grain and oils—Determination of the difference between actual and theoretical content of triacylglycerols with ECN 42

Issued on:2019-05-10
Implemented on:2019-12-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $250.00
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Standard No:	GB/T 37512-2019
Document status:	VALID
Title in English:	Inspection of grain and oils—Determination of the difference between actual and theoretical content of triacylglycerols with ECN 42
Title in Chinese:	粮油检验实际与理论ECN42甘三酯含量差值的测定
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2019-05-10
Implemented on:	2019-12-01
ICS Classification:	67.040-food products in general
Professional Classification:	GB-National Standard
Related Keywords:	theoretical ecn42 triglyceride content theoretical content oils determination ecn42 triglyceride content difference between actual
Related Topics:	Peak area content actual Peak area + content 3. Conclusions and recommendations Peak Area++ Content Theoretical content Glycerin detection International testing three-bar method Theoretical content Rapid detection of glycerol content Grain and Oil Inspection and Analysis Triester content Triglycerides Forecast vs Actual Glycerin content detection Rated output and actual output Measurement Theory Experiment GBT37512 GB/T 37512-2019 Triglyceride content in various oils Glycerin refractive parallax detection Experimental principles for the preparation and detection of deionized water Determination experiment of pA2 value What the standard values actually mean Standard deviation between theoretical value and experimental value International oil product water content testing Ester value testing Polyglycerides Detection Method

《GB/T 37512-2019粮油检验实际与理论ECN42甘三酯含量差值的测定》由TC270（全国粮油标准化技术委员会）归口，TC270SC2（全国粮油标准化技术委员会油料及油脂分会）执行，主管部门为国家粮食和物资储备局。

Introduction

National Standard of the People's Republic of China GB/T 37512—2019

Determination of the Difference Between Actual and Theoretical ECN42 Triglyceride Content in Grain and Oil Inspection

1. Standard Background and Technical Evolution Analysis

This standard was drafted in accordance with the rules given in GB/T1.1-2009, proposed by the State Administration of Grain and Material Reserves, and managed by the National Technical Committee for Grain and Oil Standardization (SAC/TC 270). This standard aims to determine whether olive oil is adulterated with other oils by measuring the difference between actual and theoretical ECN42 triglyceride content.

2. Experimental instruments and equipment

Serial number	Instrument name	Specifications and models	Quantity
1	Round bottom flask	250mL and 500mL	Multiple
2	High performance liquid chromatograph	Equipped with column temperature control device	1 unit
3	Gas chromatograph	Differential refractometer, with a maximum sensitivity of at least 10^{-4}	1 unit

3. Experimental steps

The experiment is mainly divided into two parts: sample preparation and HPLC analysis:

Sample preparation:Includes two methods: traditional column chromatography purification and solid phase extraction (SPE) purification.
HPLC analysis:After preparing the sample solution, the ECN42 triglyceride content is determined by HPLC.

4. Key data interpretation

When calculating the molar fraction of fatty acids, the peak area percentage measured by gas chromatography needs to be converted into the amount of substance and normalized. For example, the molar fractions of saturated fatty acids (SFA) and unsaturated fatty acids (UFA) at different sites can be obtained by formulas (2) and (3).

5. Standard comparative analysis

Project	High performance liquid chromatography (HPLC)	Gas chromatography (GC)
Scope of application	Direct determination of triglyceride content	Calculation of theoretical value through fatty acid composition
Sensitivity	High sensitivity, suitable for microanalysis	Applicable to large batches of samples

6. Implementation suggestions

To ensure the accuracy of the test results:

The laboratory should be equipped with professional chromatographic equipment and calibrated regularly.
The operator needs to undergo professional training and be familiar with sample pretreatment and instrument operation steps.
It is recommended that a long-term quality control program be established, including repeatability and reproducibility testing.

More samples: NB/T 47013.2-2015 DL/T 5154-2012 JJG 21-2008

Sample only — not a preview of GB/T 37512-2019