GB/T 39730-2020 in English

VALID

General requirements for cell counting—Flow cytometry

Issued on:2020-12-14
Implemented on:2021-07-01
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $160.00
$156.00

Standard No:	GB/T 39730-2020
Document status:	VALID
Title in English:	General requirements for cell counting—Flow cytometry
Title in Chinese:	细胞计数通用要求流式细胞测定法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2020-12-14
Implemented on:	2021-07-01
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	C27-Biological agent and blood products
Professional Classification:	GB-National Standard
Related Keywords:	flow cytometry introduction standard overview flow cytometry technology flow cytometer volume method definition traceability standards cell cell concentration determination
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《GB/T 39730-2020细胞计数通用要求流式细胞测定法》由424-nrccrm（国家标准物质研究中心）归口，主管部门为国家市场监督管理总局。

Introduction

Standard Overview and Technical Background

GB/T39730-2020, as my country's first national standard specifically for flow cytometry technology, was issued in December 2020 and officially implemented in July 2021. The standard was formulated by the China Institute of Metrology and filled the gap in my country's standardization of cell counting methods.

Definition of core terms

Terms	Key points of definition	Traceability standards
Cell counting	Measurement process of cell number	ISO20391-1:2018
Certified reference materials	Characteristic quantity values with uncertainty and traceability	JJF1001-2018
Measurement precision	The degree of consistency of repeated measurement results	JJF1001-2018

Detailed explanation of the principle of the method

The standard specifies two core methods:

Counting standard microsphere method: Calculate the concentration by detecting the ratio of target cells to microspheres through flow cytometer
Volume method: Directly measure the number of cells in a known volume

Key technical parameter requirements:

Cell signal collection volume ≥ 10,000
Microsphere collection volume ≥ 1,000
Voltage adjustment needs to make the signal located in the middle of the scatter plot

Instrument and equipment specifications

The standard puts forward clear requirements for flow cytometer:

Case study: A laboratory used an uncalibrated micropipette which resulted in a 15% deviation in cell concentration determination. After regular calibration according to standard requirements, the deviation was reduced to less than 3%.

Implementation Suggestions

Key Points for Quality Control

Establish a daily startup calibration system
Perform method performance verification (precision, accuracy, etc.) on a regular basis
Implement standardized training for operators

Solutions to Common Problems

Problem phenomenon	Possible cause	Solution measures
Cell aggregation	Vortex speed is too high	Adjust to ≤3000r/min
Fluorescence signal offset	Improper voltage setting	Adjust according to standard Figure 2

More samples: QC/T 942-2021 YD/T 3934-2021 JGJ 94-2008

Sample only — not a preview of GB/T 39730-2020

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