GB/T 41895-2022 in English

VALID

Determination for DNA viruses of cell—MNP marker method

Issued on:2022-10-12
Implemented on:2022-10-12
File Format:PDF
Delivery:Via email within 1~3 business days

Price(USD)： $220.00
$214.00

Standard No:	GB/T 41895-2022
Document status:	VALID
Title in English:	Determination for DNA viruses of cell—MNP marker method
Title in Chinese:	细胞中DNA病毒测定 MNP标记法
Language:	English
File Format:	Electronic (PDF)
Delivery:	Via email within 1~3 business days
Issued on:	2022-10-12
Implemented on:	2022-10-12
ICS Classification:	07.080-Biology. Botany. Zoology
Chinese Classification:	A40-Basic Subject in general
Professional Classification:	GB-National Standard
Related Keywords:	cell mnp marker method introduction analysis amplification internal control dna detection key quality control points dna viruses quality control
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in dna intermediate progenitor cells cell bacterial cell gel cytotoxicity cells are fixed mixed cell method tuft cells cell fixation sheet cell english dna committed cells Secondary cells virus in cultured cells cell culture virus method cell fixation plate cell dual localization Stem cells cellular calcium neutrophils cytofluorescent virus flow cytometry multi-label how cells are fixed dna qualitative method dna determination ldh in cells marker pcr m2 cells core cell Mycoplasma detection in cells virus cell fluorescence viral flow cytometry fixed cells ancient chinese cells dna+tm value cellular means detectable in recipient cells Cell fixation and prefixation cell pool cellular virus-like how to detect single stranded dna in cells cell if intermediate b cells Cells and Cell Engineering multiplex library viral centrosome How to detect virus in cells Cytotoxicity assay method measure dna marker dna taste cells cell + version cell marker cytotoxic mtt+ How to measure animal cells How to measure primary cell assay How to measure a cell line How to measure the cell line CMV antibody cell core Smart cells cellular virus fluorescence single cell quantification Cytometry t virus Transient labeling of cells core cell tm value in dna T4 cells GBT41895 GB/T 41895-2022 labeled stem cells Acetone cell fixation method Counter titration in virus neutralization experiments live cell labeling plasmablast b cells Determination of virus titer by cytopathic method suspension cell virus Fluorescence labeling cytoskeleton cd34+ cells dmso cytotoxicity highfive cells huh7 cells NS0 cells SP20 cells plasma cell surface markers Cytotoxic T cell mechanism of action Three methods of cell fixation Three methods of cell cycle determination primary podocytopathy DMSO toxicity to cells m0 cells The cells in the well plate are all in the middle Single cell memory t cell marker mtt cytotoxicity steps adherent cells plasma cell disease Cell preservation solution won the bid Cytotoxic T cell differentiation process Cytotoxic CT hela cells clump Cytotoxicity determination Quantitative methods for detecting specific cells Cell fixation method Fixed cell method Cell immobilization methods Viral packaging cell lysis method Acetone fixed cell method Cell serine determination method membrane cytotoxicity Cytotoxicity grading Suspension cell virus infection method Cell phototoxicity Cell organelle fluorescent labeling method Cell fixation method Neutrophil function measurement method Cytological assays Suspension cell inoculation method Methods to label viruses with isotopes Experimental Method for Cell Thermal Displacement Determination How to measure cell growth curve Methods for cell transfection with lentivirus Standard cell viability assay Methanol fixed cell method Cell smear fixation method Methods for detecting amino acids in cells Methods of fixing cells Cellular co-localization methods Animal cell fixation methods There are several ways to fix cells Cell thermal shift assay experimental method Cell growth curve measurement method

《GB/T 41895-2022细胞中DNA病毒测定 MNP标记法》由TC387（全国生化检测标准化技术委员会）归口，主管部门为国家标准化管理委员会。

Introduction

Analysis of the core technological innovation of the method

This method adopts multi-nucleotide polymorphism (MNP) labeling technology and relies on the second-generation high-throughput sequencing platform to achieve the following key breakthroughs:

Comparison of technological innovation (technical module/conventional PCR, detection sensitivity)

Technical dimension	MNP labeling method	Traditional qPCR
Maximum detection variety	9 viruses	Single target
Sensitivity (LOD)	10 copies/reaction	10²~10³
Comparison of refund points	Identification of multiple syndromes	Determination of single pathogen

Experimental equipment and sample specifications

Key technical equipment includes:

PCR amplification instrument: professional model with 30-second rapid temperature rise and fall
High-throughput sequencer: Minimum BioMEMS microfluidic chip system

Test sample requirements:

A260/A280 ∈ [1.7,1.9] A260/A230 > 2.0

Analysis of key quality control points

Sequencing quality control threshold

Quality control indicators	Conversion conditions	Handling measures
＜50Mb data	Construction failure	7.3 stage reanalysis
50-100Mb	Abnormal samples	DNA repeated extraction and amplification
Internal control DNA detection sites	>=17	Otherwise clearly out of control

Clinical diagnosis application scenarios

Applicable fields include virus clearance evaluation after vaccination, blood product cell bank testing, etc.

EB virus determination rules:

Clear detection when overlap ≥3 marker sites (47 specific probes) and signal-to-noise ratio ≥10

False positive prevention mechanism:

Non-specific amplification signals are filtered out by internal control flux negative control to reach 2×10⁻⁴Pharmacological level elimination

More samples: GB 18306-2015 QC/T 942-2021 JJG 21-2008

Sample only — not a preview of GB/T 41895-2022

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